Kofanova Olga A, Betsou Fotini
Integrated Biobank of Luxembourg (IBBL) , Luxembourg .
Biopreserv Biobank. 2013 Oct;11(5):316-8. doi: 10.1089/bio.2013.0031.
Proteomic research requires high-quality, standardized samples. Quality control (QC) biomarkers, which are sensitive to the collection, processing or storage conditions, would be useful tools to identify compromised samples. This study evaluates the usefulness of renal lithostatine as a QC tool for urine sample processing in daily biobank work. Four factors (pre-analytical variations) were examined for their effect on renal lithostatine as measured by ELISA: time from sample collection to centrifugation, number of specimen freeze-thaw cycles, specimen preservation with protease inhibitors, and the inclusion or exclusion of urinary sediment.
蛋白质组学研究需要高质量、标准化的样本。质量控制（QC）生物标志物对样本的采集、处理或储存条件敏感，将是识别受损样本的有用工具。本研究评估了肾抑素作为日常生物样本库工作中尿液样本处理质量控制工具的实用性。通过酶联免疫吸附测定（ELISA）检测了四个因素（分析前变量）对肾抑素的影响：从样本采集到离心的时间、样本冻融循环次数、使用蛋白酶抑制剂保存样本以及是否包含尿沉渣。
