Palacios A, Catalá A
Cátedra de Bioquímica, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Argentina.
Arch Int Physiol Biochim. 1989 Dec;97(6):441-6. doi: 10.3109/13813458909075075.
Cytosol obtained from bovine intestinal mucosa, contains two protein fractions that bind sulfobromophthalein and are able to remove [1-14C] palmitic acid from microsomal membranes. The high molecular weight protein fraction (F1) increases the binding of sulfobromophthalein 2 and 8 times respectively after heating at 60 degrees C during 5 min or delipidation. These changes do not correlate with the rate of palmitic acid removal from microsomes. F1 native or delipidated is more efficient than the low molecular weight protein (F2) on the removal of [1-14C]palmitic acid from microsomes. Two protein fractions DE-I and DE-II obtained from F1 by DEAE-cellulose chromatography have palmitic acid- and sulfobromophthalein-binding capacities respectively.
从牛肠黏膜获得的胞质溶胶含有两种与磺溴酞结合的蛋白质组分，并且能够从微粒体膜中去除[1-¹⁴C]棕榈酸。高分子量蛋白质组分（F1）在60℃加热5分钟或脱脂后，分别使磺溴酞的结合增加2倍和8倍。这些变化与微粒体中棕榈酸的去除速率无关。天然或脱脂的F1在从微粒体中去除[1-¹⁴C]棕榈酸方面比低分子量蛋白质（F2）更有效。通过DEAE-纤维素色谱从F1获得的两种蛋白质组分DE-I和DE-II分别具有棕榈酸和磺溴酞结合能力。
