脂肪酸结合蛋白与微粒体的相互作用：棕榈酸和视黄酯的去除
Interaction of fatty acid binding protein with microsomes: removal of palmitic acid and retinyl esters.
作者信息
Zanetti R, Catalá A
机构信息
Cátedra de Bioquímica, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Argentina.
出版信息
Arch Int Physiol Biochim. 1990 Aug;98(4):173-7. doi: 10.3109/13813459009113975.
[14C] palmitic acid or [3H] retinyl esters incorporated in microsomal membranes were removed by a cytosolic fraction enriched in fatty acid binding protein. When mouse liver cytosol was fractionated by 70% ammonium sulphate, a precipitate and a soluble fraction were obtained. The soluble fraction containing the fatty acid binding protein was able to remove from microsomal membranes, [14C] palmitic acid or [3H] retinyl esters, whereas the precipitate fraction had no removal capacity. Retinoid analysis indicated that 70% ammonium sulphate soluble fraction was enriched in endogenous retinyl esters with regard to cytosol or 70% ammonium sulphate precipitate fraction.
掺入微粒体膜中的[14C]棕榈酸或[3H]视黄酯可被富含脂肪酸结合蛋白的胞质部分去除。当用70%硫酸铵对小鼠肝脏胞质进行分级分离时，得到了一个沉淀部分和一个可溶部分。含有脂肪酸结合蛋白的可溶部分能够从小微粒体膜中去除[14C]棕榈酸或[3H]视黄酯，而沉淀部分没有去除能力。类维生素A分析表明，就胞质溶胶或70%硫酸铵沉淀部分而言，70%硫酸铵可溶部分富含内源性视黄酯。
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