Watanabe Kazuyuki, Larsson Karin, Rydevik Björn, Konno Shin-Ichi, Nordborg Claes, Olmarker Kjell
Department of Orthopaedics, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden ; Department of Orthopaedic Surgery, Fukushima Medical University, School of Medicine, Fukushima, Japan.
Department of Orthopaedics, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Open Orthop J. 2014 Apr 4;8:69-73. doi: 10.2174/1874325001408010069. eCollection 2014.
It has been assumed that nucleus pulposus-induced activation of the dorsal root ganglion (DRG) may be related to an activation of sodium channels in the DRG neurons. In this study we assessed the expression of Nav 1.8 and Nav 1.9 following disc puncture.
Thirty female Sprague-Dawley rats were used. The L4/L5 disc was punctured by a needle (n=12) and compared to a sham group without disc puncture (n=12) and a naive group (n=6). At day 1 and 7, sections of the left L4 DRG were immunostained with anti-Nav 1.8 and Nav 1.9 antibodies.
At day 1 after surgery, both Nav 1.8-IR neurons and Nav 1.9-IR neurons were significantly increased in the disc puncture group compared to the sham and naive groups (p<0.05), but not at day 7.
The findings in the present study demonstrate a neuronal mechanism that may be of importance in the pathophysiology of sciatic pain in disc herniation.
据推测，髓核诱导的背根神经节（DRG）激活可能与DRG神经元中钠通道的激活有关。在本研究中，我们评估了椎间盘穿刺后Nav 1.8和Nav 1.9的表达。
使用30只雌性Sprague-Dawley大鼠。用针穿刺L4/L5椎间盘（n = 12），并与未进行椎间盘穿刺的假手术组（n = 12）和未处理组（n = 6）进行比较。在第1天和第7天，用抗Nav 1.8和Nav 1.9抗体对左侧L4 DRG切片进行免疫染色。
术后第1天，与假手术组和未处理组相比，椎间盘穿刺组中Nav 1.8免疫反应性（IR）神经元和Nav 1.9-IR神经元均显著增加（p<0.05），但在第7天未出现这种情况。
本研究结果表明了一种神经元机制，该机制可能在椎间盘突出症所致坐骨神经痛的病理生理学中具有重要意义。
