Papke R L, Boulter J, Patrick J, Heinemann S
Molecular Neurobiology Laboratory, Salk Institute, San Diego, California 92138.
Neuron. 1989 Nov;3(5):589-96. doi: 10.1016/0896-6273(89)90269-9.
The neuronal nicotinic acetylcholine receptor subunits alpha 2, alpha 3, and alpha 4 form functional receptors with the beta 2 subunit. Each of these subunit combinations shows two distinct open states (referred to as primary and secondary). The primary open states of alpha 2 beta 2, alpha 3 beta 2, and alpha 4 beta 2 receptors were 33.6 +/- 1.8 pS, 15.4 +/- 0.8 pS, and 13.3 +/- 1.5 pS, respectively. The open times of the alpha 3 beta 2 primary open state were significantly longer than the open times of the other primary conductance states. The secondary open states of alpha 2 beta 2 and alpha 3 beta 2 were 15.5 +/- 1.3 pS and 5.1 +/- 0.4 pS, respectively. Secondary open states were seen infrequently with alpha 4 beta 2. Oocytes injected with alpha 2 RNA and a 9-fold excess of beta 2 RNA showed an enhanced expression of the secondary open state.
神经元烟碱型乙酰胆碱受体亚基α2、α3和α4与β2亚基形成功能性受体。这些亚基组合中的每一种都表现出两种不同的开放状态（称为初级和次级）。α2β2、α3β2和α4β2受体的初级开放状态分别为33.6±1.8 pS、15.4±0.8 pS和13.3±1.5 pS。α3β2初级开放状态的开放时间明显长于其他初级电导状态的开放时间。α2β2和α3β2的次级开放状态分别为15.5±1.3 pS和5.1±0.4 pS。α4β2很少出现次级开放状态。注射α2 RNA和9倍过量β2 RNA的卵母细胞表现出次级开放状态表达增强。
