Hagg T, Muir D, Engvall E, Varon S, Manthorpe M
Department of Biology, University of California, San Diego, La Jolla 92093.
Neuron. 1989 Dec;3(6):721-32. doi: 10.1016/0896-6273(89)90241-9.
Using several antibodies against rat or human laminin and an avidin-biotin immunocytochemical protocol, laminin-like immunoreactivity was detectable in the rat nervous system in expected locations, i.e., associated with blood vessels and reactive astrocytes. However, laminin staining was also abundantly present within neuronal cell bodies in most parts of the developing and adult rat CNS. Medial septum neuronal immunoreactivity was lost after septo-hippocampal disconnection, but could be preserved or even restored by intraventricular administration of nerve growth factor. Thus, at least for medial septum neurons, this laminin-like molecule can be accumulated or produced independent of direct hippocampal (target) contact. It remains to be determined whether CNS neuronal "laminin" processes activities similar to those found for laminin in vitro.
使用几种针对大鼠或人类层粘连蛋白的抗体以及抗生物素蛋白-生物素免疫细胞化学方法，在大鼠神经系统的预期位置可检测到层粘连蛋白样免疫反应性，即与血管和反应性星形胶质细胞相关。然而，在发育中和成年大鼠中枢神经系统的大部分区域，神经元细胞体内也大量存在层粘连蛋白染色。海马-隔区切断后，内侧隔区神经元的免疫反应性消失，但通过脑室内给予神经生长因子可使其保留甚至恢复。因此，至少对于内侧隔区神经元而言，这种层粘连蛋白样分子可以独立于直接的海马（靶标）接触而积累或产生。中枢神经系统神经元“层粘连蛋白”是否具有与体外发现的层粘连蛋白类似的活性仍有待确定。
