Xue Qi-Ming, Huang Lu, Pan Hui, Li Ning
Zhongguo Zhen Jiu. 2014 Mar;34(3):267-71.
To explore the mechanism of electroacupuncture at Zusanli (ST 36) on regulation of intestinal inflammatory reaction in rats with acute pancreatitis.
Fifty-four SD rats were randomly divided into a severe acute pancreatitis (SAP) group, an electroacupuncture (EA) group and a sham-operation (SO) group, 18 cases in each group. 3.5% sodium cholate was used to made SAP model by retrograde injecting in cholangiopancreatic duct. After the success of model making, the EA group was treated with EA at bilateral Zusanli (ST 36) for 30 min. The SO group and the SAP group were fixed at the same time for 30 min without treatment. All the rats were killed at 3 h, 6 h and 12 h after modeling in batches. The pathological changes of pancreatic tissue and intestinal epithelium were observed, and the expression of small intestinal occludin protein and nuclear factor kappa-B (NF-kappaB) were detected by immunohistochemical SP method.
The pathologic score and the expression of small intestinal NF-kappaB p65 at 3 h, 6 h and 12 h after modeling in the EA group and the SO group were significantly lower than those in the SAP group (all P < 0.05), and the expression of small intestinal occludin protein in the EA group and the SO group were significantly higher than that in the SAP group (all P < 0.05).
Electroacupuncture at Zusanli (ST 36) can alleviate pancreatic injury by reducing the expression of NF-kappaB p65 and enhancing the expression of occludin protein in the intestinal epithelium in the SAP model rats.
探讨电针足三里（ST 36）对急性胰腺炎大鼠肠道炎症反应的调节机制。
将54只SD大鼠随机分为重症急性胰腺炎（SAP）组、电针（EA）组和假手术（SO）组，每组18只。采用3.5%胆酸钠经胆胰管逆行注射制备SAP模型。造模成功后，EA组于双侧足三里（ST 36）行电针治疗30分钟。SO组和SAP组同时固定30分钟，未予处理。造模后3小时、6小时和12小时分批处死所有大鼠。观察胰腺组织和肠上皮的病理变化，采用免疫组织化学SP法检测小肠闭锁蛋白和核因子κB（NF-κB）的表达。
造模后3小时、6小时和12小时，EA组和SO组的病理评分及小肠NF-κB p65表达均显著低于SAP组（均P<0.05），EA组和SO组小肠闭锁蛋白的表达均显著高于SAP组（均P<0.05）。
电针足三里（ST 36）可通过降低SAP模型大鼠小肠上皮NF-κB p65的表达和增强闭锁蛋白的表达来减轻胰腺损伤。
