Vandeputte D F, Ameloot P C, Cleymaet R, Coomans D, Van Grieken R E
Department of Chemistry, University of Antwerp, Belgium.
Biol Trace Elem Res. 1989;23:133-44. doi: 10.1007/BF02917184.
Trace elements can influence dental health, possibly by altering tooth resistance during preeruptive development. Therefore, it was investigated whether lead and fluoride would be incorporated into the calcifying matrices or the cellular parts of tooth germs in vitro. Using laser microprobe mass analysis, the localization of lead and fluoride was studied in the different layers or tooth germs that had been cultured in a medium to which PbCl2 of NaF had been added in different concentrations. Both elements could only be detected in the dentine layer. Hence, the enamel organ in the secretory stage of tooth development excludes lead and fluoride from the enamel, even when enamel formation by the ameloblasts is visibly disturbed. Furthermore, there seemed to be a process of saturation in the accumulation of lead and fluoride in the dentine.
微量元素可能通过改变牙齿萌出前发育过程中的牙齿抵抗力来影响牙齿健康。因此，研究了铅和氟是否会在体外被整合到牙胚的钙化基质或细胞部分中。使用激光微探针质谱分析，研究了在添加了不同浓度的PbCl2或NaF的培养基中培养的不同层或牙胚中铅和氟的定位。两种元素仅在牙本质层中被检测到。因此，在牙齿发育的分泌阶段，成釉器将铅和氟排除在牙釉质之外，即使成釉细胞形成牙釉质的过程明显受到干扰。此外，牙本质中铅和氟的积累似乎存在一个饱和过程。
