Lyaruu D M, de Jong M, Bronckers A L, Wöltgens J H
Department of Oral Cell Biology, School of Dentistry, Vrije Universiteit, Amsterdam, The Netherlands.
Arch Oral Biol. 1987;32(2):107-15. doi: 10.1016/0003-9969(87)90053-7.
The recovery of mineralization capacity of fluorotic enamel matrix was investigated in 3-day-old hamster first molar tooth germs already pre-exposed in organ culture to 10 parts/10(6) F- for 24 h during the secretory phase. The germs were then cultured for another 24 h in a fresh medium without F-. The unmineralized fluorotic enamel matrix secreted in vitro eventually mineralized in the absence of F- but the orientation of the crystals compared to those in the fluorotic enamel was disturbed, especially in the younger regions of the enamel nearest cervical-loop in which the underlaying fluorotic enamel was most hypermineralized; but least disturbed in the more mature parts of the enamel organ in which the fluorotic enamel was less hypermineralized. The subsequent culture in F(-)-free medium did not abolish or reduce the degree of hypermineralization induced by F- treatment during the initial 24 h of culture. It seems that in vitro the inhibitory effect of F- on enamel matrix mineralization during the secretory phase is completely reversible when the ion is removed from the matrix environment, i.e. F(-)-induced synthesis and secretion of defective enamel matrix is not the cause of the lack of matrix mineralization. The F(-)-induced hypermineralization seems to be irreversible.
研究了在分泌期于器官培养中预先暴露于10份/10⁶氟离子（F⁻）24小时的3日龄仓鼠第一磨牙牙胚中氟斑牙釉质基质矿化能力的恢复情况。然后将牙胚在不含F⁻的新鲜培养基中再培养24小时。体外分泌的未矿化氟斑牙釉质基质最终在无F⁻的情况下矿化，但与氟斑牙釉质中的晶体相比，晶体的取向受到干扰，尤其是在釉质最靠近颈环的较年轻区域，其下方的氟斑牙釉质矿化程度最高；而在氟斑牙釉质矿化程度较低的牙釉器较成熟部分，干扰最小。在不含F⁻的培养基中随后的培养并未消除或降低在培养最初24小时期间F⁻处理诱导的矿化过度程度。似乎在体外，当离子从基质环境中去除时，F⁻在分泌期对釉质基质矿化的抑制作用是完全可逆的，即F⁻诱导的有缺陷釉质基质的合成和分泌不是基质矿化缺乏的原因。F⁻诱导的矿化过度似乎是不可逆的。
