微流控芯片上 129Xe 的光极化和 NMR 检测。

Optical hyperpolarization and NMR detection of 129Xe on a microfluidic chip.

机构信息

1] National Institute of Standards and Technology, 325 Broadway, Boulder, Colorado 80305, USA [2] Department of Physics, University of Colorado at Boulder, Boulder, Colorado 80309, USA.

1] Materials Science Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720-7300, USA [2] Department of Chemistry, University of California at Berkeley, Berkeley, California 94720, USA.

出版信息

Nat Commun. 2014 May 20;5:3908. doi: 10.1038/ncomms4908.

DOI:10.1038/ncomms4908

PMID:24844396

Abstract

Optically hyperpolarized (129)Xe gas has become a powerful contrast agent in nuclear magnetic resonance (NMR) spectroscopy and imaging, with applications ranging from studies of the human lung to the targeted detection of biomolecules. Equally attractive is its potential use to enhance the sensitivity of microfluidic NMR experiments, in which small sample volumes yield poor sensitivity. Unfortunately, most (129)Xe polarization systems are large and non-portable. Here we present a microfabricated chip that optically polarizes (129)Xe gas. We have achieved (129)Xe polarizations >0.5% at flow rates of several microlitres per second, compatible with typical microfluidic applications. We employ in situ optical magnetometry to sensitively detect and characterize the (129)Xe polarization at magnetic fields of 1 μT. We construct the device using standard microfabrication techniques, which will facilitate its integration with existing microfluidic platforms. This device may enable the implementation of highly sensitive (129)Xe NMR in compact, low-cost, portable devices.

摘要

光极化的 (129)Xe 气体已成为磁共振（NMR）光谱和成像中的一种强大对比剂，其应用范围从人类肺部研究到生物分子的靶向检测。同样吸引人的是，它有可能提高微流控 NMR 实验的灵敏度，因为小体积的样品会导致灵敏度降低。不幸的是，大多数 (129)Xe 极化系统体积庞大且不便携。在这里，我们展示了一种微加工芯片，可以对 (129)Xe 气体进行光极化。我们已经实现了 >0.5%的 (129)Xe 极化，流速为每秒几微升，与典型的微流控应用兼容。我们采用原位光学磁强计在 1 μT 的磁场下灵敏地检测和表征 (129)Xe 极化。我们使用标准的微加工技术构建该设备，这将便于其与现有的微流控平台集成。该设备可以实现紧凑、低成本、便携式设备中高灵敏度的 (129)Xe NMR。