Luo Hongwei, Duan Xinbin, Liu Shaoping, Chen Daqing
Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, No.8, 1st Wudayuan Road, East Lake Hi-Tech Development Zone, Wuhan, 430223, Hubei, People's Republic of China.
Fish Physiol Biochem. 2014 Oct;40(5):1521-32. doi: 10.1007/s10695-014-9945-4. Epub 2014 May 21.
The study assessed the effects of surgically implanted dummy ultrasonic transmitters on physiological response of bighead carp Hypophthalmichthys nobilis in April 2011. Before the surgery, 15 blood samples were extracted randomly from 195 bighead carp samples, and then the rest of the fish were divided into three groups: (1) control group, handing but no tagging, (2) sham group, surgical procedure without implantation of transmitter and (3) surgery group, surgical implantation of transmitters. In 3 h, 24 h, 7 days and 14 days after surgery, 15 fish were extracted randomly from the three groups, respectively, for sampling. Then the plasma samples were analyzed, and physiological measures of stress response (cortisol, glucose), tissue damage [alanine aminotransferase (ALT) and aspartate aminotransferase (AST)] and nutritional status [total protein, globulin, albumin, triglyceride, cholesterol, and alkaline phosphatase (ALP)] were compared. The result showed that there was no significant difference between sham and surgery groups in 3 h, 24 h, 7 days and 14 days after surgery. When compared to the control group, there were significant increases in concentrations of plasma cortisol, glucose, ALT, AST, total protein and globulin of sham and surgery groups in 3 h after surgery. After 24 h, the levels of plasma cortisol, ALT, AST, total protein, globulin and ALP were elevated in both sham and surgery groups, whereas the levels of plasma glucose had declined to normal level and plasma albumin, cholesterol and triglyceride were significantly decreased in both sham and surgery groups. After 7 days, the levels of plasma glucose, albumin and cholesterol continued to decline, while the level of plasma ALT, globulin and ALP had declined but still remained higher for sham and surgery groups than control group; however, the plasma total protein level had returned to normal. After 14 days, there was no significant difference between the three groups. The above results showed that surgical implantation of ultrasonic transmitters had indeed caused significantly negative effects on the physiological response of bighead carp. However, all the negative influences on stress response, tissue damage and nutritional status had disappeared in 14 days after surgery, and the presence of transmitters had no significant negative impact. Therefore, the bighead carp should be temporary cultured at least 14 days for the recovery of physiological response and then released into the free water for the ultrasonic telemetry research.
2011年4月，该研究评估了手术植入的模拟超声发射器对鳙鱼（Hypophthalmichthys nobilis）生理反应的影响。手术前，从195尾鳙鱼样本中随机抽取15份血样，然后将其余的鱼分为三组：（1）对照组，仅进行处理但不标记；（2）假手术组，进行手术操作但不植入发射器；（3）手术组，手术植入发射器。术后3小时、24小时、7天和14天，分别从三组中随机抽取15尾鱼进行采样。然后对血浆样本进行分析，并比较应激反应（皮质醇、葡萄糖）、组织损伤[丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）]以及营养状况[总蛋白、球蛋白、白蛋白、甘油三酯、胆固醇和碱性磷酸酶（ALP）]的生理指标。结果显示，术后3小时、24小时、7天和14天，假手术组和手术组之间无显著差异。与对照组相比，假手术组和手术组术后3小时血浆皮质醇、葡萄糖、ALT、AST、总蛋白和球蛋白浓度显著升高。24小时后，假手术组和手术组血浆皮质醇、ALT、AST、总蛋白、球蛋白和ALP水平均升高，而血浆葡萄糖水平已降至正常水平，假手术组和手术组血浆白蛋白、胆固醇和甘油三酯显著降低。7天后，血浆葡萄糖、白蛋白和胆固醇水平持续下降，而血浆ALT、球蛋白和ALP水平有所下降，但假手术组和手术组仍高于对照组；然而，血浆总蛋白水平已恢复正常。14天后，三组之间无显著差异。上述结果表明，超声发射器的手术植入确实对鳙鱼的生理反应产生了显著的负面影响。然而，术后14天，对应激反应、组织损伤和营养状况的所有负面影响均消失，发射器的存在没有显著的负面影响。因此，鳙鱼应至少暂养14天以恢复生理反应，然后放入自由水域进行超声遥测研究。
