Ning Chenxi, Su Erzheng, Tian Yanjun, Wei Dongzhi
State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, PR China.
Enzyme and Fermentation Technology Laboratory, College of Light Industry Science and Engineering, Nanjing Forestry University, Nanjing 210037, PR China.
J Biotechnol. 2014 Aug 20;184:7-10. doi: 10.1016/j.jbiotec.2014.05.004. Epub 2014 May 17.
An alternative strategy for cofactor regeneration in the synthesis of valuable chiral alcohols catalyzed by ketoreductases was developed. combi-CLEAs of ketoreductase and d-glucose dehydrogenase enabled the repeated and effective conversion of substrate ethyl 4-chloro-3-oxobutanoate (COBE) with several superiorities. Wide application of this strategy in production of various chiral alcohols could be expected in the future for its high efficiency with low cost.
开发了一种在酮还原酶催化合成有价值的手性醇过程中进行辅因子再生的替代策略。酮还原酶和d-葡萄糖脱氢酶的组合交联酶聚集体能够对底物4-氯-3-氧代丁酸乙酯（COBE）进行重复有效的转化，并具有若干优势。由于该策略高效且低成本，预计未来在各种手性醇的生产中会得到广泛应用。
