College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, 700 Changcheng Road, Qingdao, 266109, China.
College of Life Sciences, Energy-Rich Compounds Production by Photosynthesis Carbon Fixation Research Center, Shandong Province Key Laboratory of Applied Mycology, Qingdao Agricultural University, 700 Changcheng Road, Qingdao, 266109, China.
World J Microbiol Biotechnol. 2018 Sep 10;34(10):141. doi: 10.1007/s11274-018-2530-8.
Nicotinamide adenine dinucleotide (NADH) and nicotinamide adenine dinucleotide phosphate (NADPH), as two kinds of well-known cofactor, are widely used in the most of enzymatic redox reactions, playing an important role in industrial catalysis. In general, supply of NAD(P)H is a major challenged factor in redox fermentation systems due to its high cost and low stability, which have stimulated the development of NADH regeneration systems in recent years. Until now, a series of NAD(P)H regeneration systems have been developed. This review focuses primarily on new approaches of NAD(P)H cofactor regeneration in the biosynthesis systems, such as single cell in vivo NADH regeneration system, double cell coupling NADH regeneration system, in vitro enzyme-coupled NADH regeneration system, microbial cell surface display NADH regeneration system. Finally, the prospect and tendency of NADH regeneration are discussed.
烟酰胺腺嘌呤二核苷酸 (NADH) 和烟酰胺腺嘌呤二核苷酸磷酸 (NADPH) 作为两种著名的辅酶，广泛应用于大多数酶促氧化还原反应中，在工业催化中发挥着重要作用。一般来说，由于 NAD(P)H 成本高且稳定性低，其供应成为氧化还原发酵系统的主要挑战因素，这刺激了近年来 NADH 再生系统的发展。到目前为止，已经开发了一系列 NAD(P)H 再生系统。本文主要集中于生物合成系统中 NAD(P)H 辅酶再生的新方法，如：单细胞体内 NADH 再生系统、双细胞偶联 NADH 再生系统、体外酶偶联 NADH 再生系统、微生物细胞表面展示 NADH 再生系统。最后，讨论了 NADH 再生的前景和趋势。
