Pandey Ashutosh, Misra Prashant, Bhambhani Sweta, Bhatia Chitra, Trivedi Prabodh Kumar
1] Council of Scientific and Industrial Research-National Botanical Research Institute, (CSIR-NBRI), Rana Pratap Marg, Lucknow-226 001, INDIA [2].
Council of Scientific and Industrial Research-National Botanical Research Institute, (CSIR-NBRI), Rana Pratap Marg, Lucknow-226 001, INDIA.
Sci Rep. 2014 May 21;4:5018. doi: 10.1038/srep05018.
Flavonoids, due to their pharmacological attributes, have recently become target molecules for metabolic engineering in commonly consumed food crops. Strategies including expression of single genes and gene pyramiding have provided only limited success, due principally to the highly branched and complex biosynthetic pathway of the flavonoids. Transcription factors have been demonstrated as an efficient tool for metabolic engineering of this pathway, but often exhibit variation in heterologous systems relative to that in the homologous system. In the present work, Arabidopsis MYB transcription factor, AtMYB111, has been expressed in tobacco to study whether this can enhance flavonoid biosynthesis in heterologous system. The results suggest that AtMYB111 expression in transgenic tobacco enhances expression of genes of the phenylpropanoid pathway leading to an elevated content of flavonols. However, dark incubation of transgenic and wild type (WT) plants down-regulated both the expression of genes as well as flavonoid content as compared to light grown plants. The study concludes that AtMYB111 can be effectively used in heterologous systems, however, light is required for its action in modulating biosynthetic pathway.
由于类黄酮具有药理特性，它们最近已成为常见食用作物代谢工程中的目标分子。包括单基因表达和基因聚合在内的策略仅取得了有限的成功，主要原因是类黄酮的生物合成途径高度分支且复杂。转录因子已被证明是该途径代谢工程的有效工具，但相对于同源系统，它们在异源系统中往往表现出差异。在本研究中，拟南芥MYB转录因子AtMYB111已在烟草中表达，以研究这是否能增强异源系统中的类黄酮生物合成。结果表明，转基因烟草中AtMYB111的表达增强了苯丙烷途径基因的表达，导致黄酮醇含量升高。然而，与光照生长的植物相比，转基因和野生型（WT）植物在黑暗中培养会下调基因表达以及类黄酮含量。该研究得出结论，AtMYB111可有效地用于异源系统，然而，光照是其调节生物合成途径所必需的。
