Zhao Li, Cao Yu, Shan Gaomeng, Zhou Jiayi, Li Xintong, Liu Peng, Wang Yansong, An Songhao, Gao Ri
College of Agricultural, Yanbian University, Yanji, Jilin, China.
Yanbian Academy of Forestry Sciences, Yanji, Jilin, China.
Front Plant Sci. 2024 Nov 1;15:1461357. doi: 10.3389/fpls.2024.1461357. eCollection 2024.
Gongju is recognized as one of the four traditional Chinese medicinal herbs, and its main constituents are chlorogenic acid (CGA) and its derivative material. CGA content in autooctoploid Gongju are considerably elevated compared with those in parental tetraploid Gongju at different flowering stages. However, the underlying molecular mechanisms governing the regulation CGA content remain poorly understood.
Therefore, we conducted integrated transcriptome and metabolome analyses of different flowering stages in autooctoploid and tetraploid Gongju to elucidate the underlying molecular mechanisms governing CGA biosynthesis.
Transcriptome analysis showed that the number of differentially expressed genes in the budding stage (BS), early flowering stage (EF), and full flowering stage (FF) of tetraploid and octoploid Gongju were 3859, 11,211, and 6837, respectively. A total of 563, 466, and 394 differential accumulated metabolites were respectively identified between the bud stages of tetraploid and octoploid Gongju (4BS vs. 8BS), between the early flowering stages of tetraploid and octoploid Gongju (4EF vs. 8EF), and the full flowering stages of tetraploid and octoploid Gongju (4FF vs. 8FF) groups. The integrated analysis of transcriptomics and metabolomics revealed that the expression of pma6460 and mws0178, which are key enzymes involved in the CGA synthesis pathway, increased during the flowering stages of octoploid Gongju relative to that of tetraploid Gongju. The expression levels of and genes associated with CGA synthesis were higher in octoploid plants than in tetraploid plants at various flowering stages. To investigate the potential regulation of transcription factors involved in CGA synthesis, we analyzed the coexpression of and with and . Results indicated that transcription factors, such as (Cluster-30519.0), (Cluster-75874.0), (Cluster-94106.0), (Cluster-71968.7), (Cluster-32024.1), (Cluster-62341.0), (Cluster-32024.8), (Cluster-60210.0), and (Cluster-90665.1) play a pivotal role in CGA synthesis regulation. The present study provides novel insights into the molecular mechanisms underlying CGA accumulation in autopolyploid Gongju.
贡菊被公认为四大传统中药材之一，其主要成分是绿原酸（CGA）及其衍生物。在不同开花阶段，同源八倍体贡菊中的CGA含量比亲本四倍体贡菊中的CGA含量显著升高。然而，调控CGA含量的潜在分子机制仍知之甚少。
因此，我们对同源八倍体和四倍体贡菊不同开花阶段进行了转录组和代谢组的综合分析，以阐明调控CGA生物合成的潜在分子机制。
转录组分析表明，四倍体和八倍体贡菊在现蕾期（BS）、初花期（EF）和盛花期（FF）的差异表达基因数量分别为3859、11211和6837个。在四倍体和八倍体贡菊的现蕾期（4BS与8BS）、初花期（4EF与8EF）以及盛花期（4FF与8FF）组之间，分别鉴定出563、466和394种差异积累代谢物。转录组学和代谢组学的综合分析表明，相对于四倍体贡菊，参与CGA合成途径的关键酶pma⁶⁴⁶⁰和mws⁰¹⁷⁸的表达在八倍体贡菊开花阶段增加。在各个开花阶段，与CGA合成相关的基因在八倍体植株中的表达水平高于四倍体植株。为了研究参与CGA合成的转录因子的潜在调控作用，我们分析了与和的共表达。结果表明，转录因子，如（Cluster-30519.0）、（Cluster-75874.0）、（Cluster-94106.0）、（Cluster-71968.7）、（Cluster-32024.1）、（Cluster-62341.0）、（Cluster-32024.8）、（Cluster-60210.0）和（Cluster-90665.1）在CGA合成调控中起关键作用。本研究为同源多倍体贡菊中CGA积累的分子机制提供了新的见解。
