Indriolo Emily, Goring Daphne R
Department of Cell & Systems Biology, University of Toronto Toronto, ON, Canada.
Department of Cell & Systems Biology, University of Toronto Toronto, ON, Canada ; Centre for the Analysis of Genome Evolution & Function, University of Toronto Toronto, ON, Canada.
Front Plant Sci. 2014 May 5;5:181. doi: 10.3389/fpls.2014.00181. eCollection 2014.
Ubiquitination plays essential roles in the regulation of many processes in plants including pollen rejection in self-incompatible species. In the Brassicaceae (mustard family), self-incompatibility drives the rejection of self-pollen by preventing pollen hydration following pollen contact with the stigmatic surface. Self-pollen is recognized by a ligand-receptor pair: the pollen S-locus cysteine rich/S-locus protein 11 (SCR/SP11) ligand and the pistil S receptor kinase (SRK). Following self-pollen contact, the SCR/SP11 ligand on the pollen surface binds to SRK on the pistil surface, and the SRK-activated signaling pathway is initiated. This pathway includes the armadillo repeat containing 1 (ARC1) protein, a member of the plant U-box (PUB) family of E3 ubiquitin ligases. ARC1 is a functional E3 ligase and is required downstream of SRK for the self-incompatibility response. This mini review highlights our recent progress in establishing ARC1's conserved role in self-pollen rejection in Brassica and Arabidopsis species and discusses future research directions in this field.
泛素化在植物许多生理过程的调控中发挥着重要作用，包括自交不亲和物种中的花粉排斥。在十字花科（芥菜科）中，自交不亲和通过阻止花粉与柱头表面接触后的水合作用来驱动对自身花粉的排斥。自身花粉由一对配体 - 受体识别：花粉S位点富含半胱氨酸/ S位点蛋白11（SCR / SP11）配体和雌蕊S受体激酶（SRK）。自身花粉接触后，花粉表面的SCR / SP11配体与雌蕊表面的SRK结合，从而启动SRK激活的信号通路。该通路包括含犰狳重复序列1（ARC1）蛋白，它是植物E3泛素连接酶的植物U-box（PUB）家族成员。ARC1是一种功能性E3连接酶，是SRK下游自交不亲和反应所必需的。这篇综述强调了我们最近在确定ARC1在芸苔属和拟南芥物种自身花粉排斥中的保守作用方面取得的进展，并讨论了该领域未来的研究方向。
