Lazăr L, Rădulescu S
Arch Roum Pathol Exp Microbiol. 1989 Oct-Dec;48(4):357-65.
By using the elective diagnosis methodology for Cryptosporidium (modified Ziehl-Neelsen and Heine stainings), 481 faeces samples from patients--children and adults--presenting acute or prolonged gastroenteritis and also from asymptomatic ones, but with professional, immunological or pharmacological risk for infection, were studied. Twelve Cryptosporidium positive cases were identified, meaning a general prevalence of 2.48% Cryptosporidium infection was detected in all investigated epidemiological groups: children, animal handlers, immunodeficient patients, immunocompetent adults with acute gastroenteritis. The risk factors hierarchy elicited the role of children collectivities attending, for acquiring the parasite in infantile cryptosporidiosis, while the opportunistic and zoonotic feature of the disease was especially present in adults' cryptosporidiosis. The assessment of cryptosporidiosis evolution, according to patients' immune status, has not allowed an obvious clinical discrepancy, since the immunocompetent subjects with professional risk had presented a prolonged oocysts excretion. The different clinico-epidemiological and parasitological features of Cryptosporidium infection are commented and the importance of applying Cryptosporidium methodology in current diagnosis practice is emphasized.
通过使用隐孢子虫的选择性诊断方法（改良齐-尼氏染色法和海涅氏染色法），对481份粪便样本进行了研究，这些样本来自患有急性或持续性肠胃炎的患者（包括儿童和成人）以及无症状但有职业、免疫或药物感染风险的人群。共鉴定出12例隐孢子虫阳性病例，这意味着在所有调查的流行病学组中，隐孢子虫感染的总体患病率为2.48%，这些流行病学组包括儿童、动物饲养员、免疫缺陷患者、患有急性肠胃炎的免疫功能正常的成年人。风险因素层次结构揭示了儿童集体活动在婴儿隐孢子虫病感染寄生虫方面的作用，而该疾病的机会性和人畜共患特征在成人隐孢子虫病中尤为明显。根据患者的免疫状态对隐孢子虫病演变进行的评估并未发现明显的临床差异，因为有职业风险的免疫功能正常的受试者出现了卵囊排泄延长的情况。文中对隐孢子虫感染的不同临床流行病学和寄生虫学特征进行了评论，并强调了在当前诊断实践中应用隐孢子虫诊断方法的重要性。
