Lazăr L, Rădulescu S
Rev Ig Bacteriol Virusol Parazitol Epidemiol Pneumoftiziol Bacteriol Virusol Parazitol Epidemiol. 1989 Oct-Dec;34(4):361-8.
With a view to optimize the etiological diagnosis of diarrhoea the authors have attempted to improve the diagnostic methodology for the parasite Cryptosporidium spp. as a current general technique, as well as a screening technique. A comparative evaluation of the various methods used for the concentration and the staining of cysts of Cryptosporidium spp. was done after quantification according to a five parameter score: electiveness, morphology, chromatic contrast, rapidity and accessibility. The importance of the screening tests in the diagnosis of cryptosporidiosis was evaluated according to indicators such as sensitivity, specificity and predictive value. The maximal general score (17) was obtained with the modified Ziehl-Neelsen stain which was both sensitive and viable, and was used as a method for the confirmation of the presence of the parasite. With regard to the screening methods rapid techniques employing methylene-blue and/or fuchsine gave indicator values between 76% and 83%. The best results were obtained when samples were enriched with the aid of concentration techniques. It is appreciated that the concomitant and repeated use of two techniques of diagnosis, a rapid one and a confirmation technique aimed at demonstrating the presence of Cryptosporidium cysts allows a competent parasitological diagnosis of cryptosporidiosis.
为优化腹泻的病因诊断，作者尝试改进作为当前常规技术及筛查技术的隐孢子虫属寄生虫的诊断方法。在根据五个参数评分（选择性、形态、色彩对比度、速度和可及性）进行量化后，对用于隐孢子虫属囊肿浓缩和染色的各种方法进行了比较评估。根据敏感性、特异性和预测值等指标评估了筛查试验在隐孢子虫病诊断中的重要性。改良齐-尼氏染色获得了最高综合评分（17分），该方法既敏感又可行，被用作确认寄生虫存在的方法。关于筛查方法，采用亚甲蓝和/或品红的快速技术的指标值在76%至83%之间。当样本借助浓缩技术进行富集时，获得了最佳结果。可以认识到，同时并重复使用两种诊断技术，一种快速技术和一种旨在证明隐孢子虫囊肿存在的确认技术，能够对隐孢子虫病进行有效的寄生虫学诊断。
