Department of Epidemiology, School of Public Health, University of Washington, Box 357236, Seattle, WA 98195, USA; International Agency for Research on Cancer, Section of Environment and Radiation, 150 Cours Albert Thomas, Lyon Cedex 08 69372, France.
Department of Pathology, Harborview Medical Center, Seattle, WA 98104, USA.
Cancer Epidemiol. 2014 Aug;38(4):369-75. doi: 10.1016/j.canep.2014.04.005. Epub 2014 May 19.
The importance of human papillomavirus (HPV) viral load in the pathogenesis of cervical cancer among HIV-infected and HIV-uninfected women has not yet been established.
In this cross-sectional study, HPV-16 viral loads were measured using previously-collected and frozen cervical swab samples from 498 HPV-16 positive Senegalese women (368 HIV-seronegative, 126 HIV-1 and/or HIV-2 seropositive). The real-time polymerase chain reaction assay was used to quantify HPV-16 E7 copy number normalized by human cellular DNA (β-actin), and viral loads were log10 transformed. Associations between HPV-16 viral load, degree of cervical abnormality, and HIV status were assessed using multinomial and linear regression methods.
Compared to women with normal cytology, the likelihood of CIN1 (ORa: 1.21, 95% CI 0.93-1.57), CIN2-3 (ORa: 2.38, 95% CI 1.72-3.29) and cancer (ORa: 2.12, 95% CI 1.52-2.96) was found to increase for each 1-unit log10 increase in HPV-16 viral load. Compared to HIV-negative women, HIV-positive women had higher average HPV-16 viral load values (βa: 0.39, 95% CI 0.03-0.75), even after accounting for degree of cervical abnormality.
In our study of women including those with cancer, HPV-16 viral load was associated with a higher likelihood of cervical abnormalities. However, substantial overlaps across categories of disease severity existed. Higher viral load among HIV-infected individuals may indicate that HIV infection influences HPV viral replication factors.
人乳头瘤病毒（HPV）病毒载量在 HIV 感染和未感染妇女宫颈癌发病机制中的重要性尚未确定。
在这项横断面研究中，使用之前收集和冷冻的 498 名 HPV-16 阳性塞内加尔妇女（368 名 HIV 血清阴性，126 名 HIV-1 和/或 HIV-2 血清阳性）的宫颈拭子样本测量 HPV-16 病毒载量。使用实时聚合酶链反应测定法对 HPV-16 E7 拷贝数进行定量，并用人类细胞 DNA（β-肌动蛋白）进行标准化，并对病毒载量进行对数转换。使用多项和线性回归方法评估 HPV-16 病毒载量、宫颈异常程度和 HIV 状态之间的关联。
与细胞学正常的妇女相比，CIN1（ORa：1.21，95%CI 0.93-1.57）、CIN2-3（ORa：2.38，95%CI 1.72-3.29）和癌症（ORa：2.12，95%CI 1.52-2.96）的可能性随着 HPV-16 病毒载量每增加 1 个单位的 log10 而增加。与 HIV 阴性妇女相比，HIV 阳性妇女的 HPV-16 病毒载量平均值较高（βa：0.39，95%CI 0.03-0.75），即使考虑到宫颈异常程度也是如此。
在包括癌症患者的女性研究中，HPV-16 病毒载量与宫颈异常的可能性增加相关。然而，疾病严重程度类别的重叠很大。HIV 感染者的病毒载量较高可能表明 HIV 感染影响 HPV 病毒复制因素。
