Rousseau Marie-Noelle Didelot, Costes Valérie, Konate Issouf, Nagot Nicolas, Foulongne Vincent, Ouedraogo Abdoulaye, Van de Perre Philippe, Mayaud Philippe, Segondy Michel
Department of Virology, Montpellier University Hospital, Montpellier, France.
J Med Virol. 2007 Jun;79(6):766-70. doi: 10.1002/jmv.20888.
The relationships between human papillomavirus type 16 (HPV 16) viral load, HPV 16 integration status, human immunodeficiency virus type 1 (HIV-1) status, and cervical cytology were studied among women enrolled in a cohort of female sex workers in Burkina Faso. The study focused on 24 HPV 16-infected women. The HPV 16 viral load in cervical samples was determined by real-time PCR. Integration ratio was estimated as the ratio between E2 and E6 genes DNA copy numbers. Integrated HPV16 viral load was defined as the product of HPV 16 viral load by the integration ratio. High HPV 16 viral load and high integration ratio were more frequent among women with squamous intraepithelial lesions compared with women with normal cytology (33% vs. 11%, and 33% vs. 0%, respectively), and among women with high-grade squamous intraepithelial lesions compared with women without high-grade squamous intraepithelial lesions (50% vs. 17%, and 50% vs. 11%, respectively). High HPV 16 DNA load, but not high integration ratio, was also more frequent among HIV-1-positive women (39% vs. 9%; and 23% vs. 18%, respectively). The absence of statistical significance of these differences might be explained by the small study sample size. High-integrated HPV 16 DNA load was significantly associated with the presence of high-grade squamous intraepithelial lesions (50% vs. 5%, P = 0.03) in univariate and multivariate analysis (adjusted odds-ratio: 19.05; 95% confidence interval (CI), 1.11-328.3, P = 0.03), but not with HIV-1 or other high-risk HPV types (HR-HPV). Integrated HPV 16 DNA load may be considered as a useful marker of high-grade cervical lesions in HPV 16-infected women.
在布基纳法索一组女性性工作者中，研究了16型人乳头瘤病毒（HPV 16）病毒载量、HPV 16整合状态、1型人类免疫缺陷病毒（HIV-1）状态与宫颈细胞学之间的关系。该研究聚焦于24名HPV 16感染女性。通过实时聚合酶链反应测定宫颈样本中的HPV 16病毒载量。整合率估计为E2和E6基因DNA拷贝数之比。整合型HPV16病毒载量定义为HPV 16病毒载量与整合率的乘积。与细胞学正常的女性相比，鳞状上皮内病变女性中高HPV 16病毒载量和高整合率更为常见（分别为33%对11%，以及33%对0%）；与无高级别鳞状上皮内病变的女性相比，高级别鳞状上皮内病变女性中高HPV 16病毒载量和高整合率也更为常见（分别为50%对17%，以及50%对11%）。HIV-1阳性女性中高HPV 16 DNA载量更为常见，但高整合率并非如此（分别为39%对9%；以及23%对18%）。这些差异缺乏统计学意义可能是由于研究样本量较小。在单变量和多变量分析中，高整合型HPV 16 DNA载量与高级别鳞状上皮内病变的存在显著相关（50%对5%，P = 0.03）（调整后的优势比：19.05；95%置信区间（CI），1.11 - 328.3，P = 0.03），但与HIV-1或其他高危型人乳头瘤病毒（HR-HPV）无关。整合型HPV 16 DNA载量可被视为HPV 16感染女性高级别宫颈病变的有用标志物。
