Gil Krzysztof, Bugajski Andrzej, Kurnik Magdalena, Thor Piotr
Department of Pathophysiology, Jagiellonian University Medical College, Krakow, Poland.
Department of Pathophysiology, Jagiellonian University Medical College, Kraków, Poland.
Folia Med Cracov. 2012;52(3-4):79-96.
There is growing evidence that vagus nerve stimulation (VNS) exerts a suppressive effect on both short- and long-term feeding in animal models. We previously showed that VNS with high-frequency (10 Hz) electrical impulses decreased food intake and body weight in rats. In the present study, we investigated the effect of VNS with a low frequency (1 Hz) on the serum lipid concentrations, feeding behavior and appetite in rats fed a high-fat diet. The levels of appetite-regulating peptides were also assessed. Adult male Wistar rats were subcutaneously implanted with a microstimulator (MS) and fed a high-fat diet throughout the entire study period (42 days). The left vagus nerve was stimulated subdiaphragmatically by rectangular electrical pulses (10 ms, 200 mV, 1 Hz, 12 h a day) generated by the MS. The daily food intake and body weight were measured each morning. At the end of the experiments, the serum glucose, cholesterol, triglycerides, low-density lipoproteins, high-density lipoproteins, ghrelin, leptin and nesfatin-1 concentrations were measured. The adipose tissue content was evaluated by the assessment of the weight of the epididymal fat pads. Chronic VNS significantly decreased food intake, body weight gain and epididymal fat pad weight. VNS also lowered the total plasma cholesterol concentrations and triglyceride levels. Finally, the serum concentrations of nesfatin-1 were elevated, leptin levels were decreased, and ghrelin levels remained unchanged after VNS. The study demonstrates that chronic electrical VNS exerts anorexigenic effects, lowering the blood concentration of lipids. Increased nesfatin-1 levels may contribute to these effects.
越来越多的证据表明，在动物模型中，迷走神经刺激（VNS）对短期和长期进食均有抑制作用。我们之前的研究表明，高频（10Hz）电脉冲刺激迷走神经可降低大鼠的食物摄入量和体重。在本研究中，我们调查了低频（1Hz）VNS对高脂饮食喂养大鼠的血脂浓度、进食行为和食欲的影响。同时还评估了食欲调节肽的水平。成年雄性Wistar大鼠皮下植入微型刺激器（MS），并在整个研究期间（42天）给予高脂饮食。通过MS产生的矩形电脉冲（10ms，200mV，1Hz，每天12小时）在膈下刺激左侧迷走神经。每天早晨测量食物摄入量和体重。实验结束时，测量血清葡萄糖、胆固醇、甘油三酯、低密度脂蛋白、高密度脂蛋白、胃饥饿素、瘦素和nesfatin-1的浓度。通过评估附睾脂肪垫的重量来评价脂肪组织含量。慢性VNS显著降低了食物摄入量、体重增加和附睾脂肪垫重量。VNS还降低了总血浆胆固醇浓度和甘油三酯水平。最后，VNS后血清nesfatin-1浓度升高，瘦素水平降低，胃饥饿素水平保持不变。该研究表明，慢性电刺激VNS具有厌食作用，可降低血脂浓度。nesfatin-1水平升高可能有助于这些作用。
