Worrall D M, Goss N H
Biotechnology Australia Pty Ltd.
Aust J Biotechnol. 1989 Jan;3(1):28-32.
Culture conditions affecting the formation of beta-galactosidase inclusion bodies in E. coli were examined. High temperature, early induction, high salt concentration and low aeration were all found to favour an increase of insoluble beta-galactosidase and the formation of visible inclusion bodies. The ratio of soluble to insoluble beta-galactosidase decreased during the course of cell growth. When assayed for beta-galactosidase activity, the inclusion bodies were enzymatically active with a specific activity of one third that of soluble beta-galactosidase. The activity remained associated with the inclusion bodies on washing with detergent and high ionic strength buffers. These results suggest that inclusion bodies can contain correctly folded protein.
研究了影响大肠杆菌中β-半乳糖苷酶包涵体形成的培养条件。发现高温、早期诱导、高盐浓度和低通气量均有利于不溶性β-半乳糖苷酶的增加和可见包涵体的形成。在细胞生长过程中，可溶性β-半乳糖苷酶与不溶性β-半乳糖苷酶的比例下降。当检测β-半乳糖苷酶活性时，包涵体具有酶活性，比活性为可溶性β-半乳糖苷酶的三分之一。在用去污剂和高离子强度缓冲液洗涤时，活性仍与包涵体相关。这些结果表明包涵体可以包含正确折叠的蛋白质。
