Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences , Beijing, China.
ACS Appl Mater Interfaces. 2014 Jun 25;6(12):9634-42. doi: 10.1021/am5020666. Epub 2014 Jun 3.
The construction of molecularly imprinted polymers on magnetic nanoparticles gives access to smart materials with dual functions of target recognition and magnetic separation. In this study, the superparamagnetic surface-molecularly imprinted nanoparticles were prepared via surface-initiated reversible addition-fragmentation chain transfer (RAFT) polymerization using ofloxacin (OFX) as template for the separation of fluoroquinolones (FQs). Benefiting from the living/controlled nature of RAFT reaction, distinct core-shell structure was successfully constructed. The highly uniform nanoscale MIP layer was homogeneously grafted on the surface of RAFT agent TTCA modified Fe3O4@SiO2 nanoparticles, which favors the fast mass transfer and rapid binding kinetics. The target binding assays demonstrate the desirable adsorption capacity and imprinting efficiency of Fe3O4@MIP. High selectivity of Fe3O4@MIP toward FQs (ofloxacin, pefloxacin, enrofloxacin, norfloxacin, and gatifloxacin) was exhibited by competitive binding assay. The Fe3O4@MIP nanoparticles were successfully applied for the direct enrichment of five FQs from human urine. The spiked human urine samples were determined and the recoveries ranging from 83.1 to 103.1% were obtained with RSD of 0.8-8.2% (n = 3). This work provides a versatile approach for the fabrication of well-defined MIP on nanomaterials for the analysis of complicated biosystems.
将分子印迹聚合物构建在磁性纳米粒子上，可以得到具有目标识别和磁分离双重功能的智能材料。在这项研究中，通过表面引发可逆加成-断裂链转移（RAFT）聚合，以氧氟沙星（OFX）为模板，制备了超顺磁性表面分子印迹纳米粒子，用于分离氟喹诺酮类（FQs）。受益于 RAFT 反应的活性/可控特性，成功构建了明显的核壳结构。高度均匀的纳米级 MIP 层均匀地接枝在 RAFT 试剂 TTCA 改性的 Fe3O4@SiO2 纳米粒子表面上，有利于快速传质和快速结合动力学。目标结合分析表明，Fe3O4@MIP 具有理想的吸附容量和印迹效率。通过竞争结合分析，Fe3O4@MIP 对 FQs（氧氟沙星、培氟沙星、恩诺沙星、诺氟沙星和加替沙星）表现出高选择性。Fe3O4@MIP 纳米粒子成功地用于直接从人尿中富集五种 FQs。对加标人尿样品进行了测定，回收率在 83.1%至 103.1%之间，相对标准偏差为 0.8%至 8.2%（n = 3）。这项工作为在纳米材料上制备具有良好定义的分子印迹聚合物用于分析复杂生物体系提供了一种通用方法。
