Ariani Marisa Dwi, Zuhrotun Ade, Manesiotis Panagiotis, Hasanah Aliya Nur
Pharmaceutical Analysis and Medicinal Chemistry Department, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang, Bandung 45463, West Java, Indonesia.
Pharmacy Biology Department, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang, Bandung 45463, West Java, Indonesia.
Polymers (Basel). 2022 Mar 29;14(7):1389. doi: 10.3390/polym14071389.
During the last few years, separation techniques using molecularly imprinted polymers (MIPs) have been developed, making breakthroughs using magnetic properties. Compared to conventional MIPs, magnetic molecularly imprinted polymers (MMIPs) have advantages in sample pretreatment due to their high specificity and selectivity towards analytes as a result of their larger specific surface areas and highly accessible specific binding sites. The techniques of isolation of active compounds from natural products usually require very long process times and low compound yields. When MMIPs are used in sample separation as Solid Phase Extraction (SPE) sorbents, the MMIPs are introduced into the dissolved sample and spread evenly, and they form bonds between the analyte and the MMIPs, which are then separated from the sample matrix using an external magnetic field. This process of separating analytes from the sample matrix makes the separation technique with MMIPs very simple and easy. This review discusses how to synthesize MMIPs, which factors must be considered in their synthesis, and their application in the separation of active compounds from natural products. MMIPs with magnetic core-shells made by co-precipitation can be a good choice for further development due to the high synthesis yield. Further optimization of the factors affecting the size and distribution of magnetic core-shell particles can obtain higher synthesis yields of MMIPs with higher adsorption capacity and selectivity. Thus, they can isolate target compounds from natural plants in high yields and purity.
在过去几年中，利用分子印迹聚合物（MIPs）的分离技术得到了发展，并在利用磁性方面取得了突破。与传统的MIPs相比，磁性分子印迹聚合物（MMIPs）由于其较大的比表面积和高度可及的特异性结合位点，对分析物具有高特异性和选择性，因而在样品预处理方面具有优势。从天然产物中分离活性化合物的技术通常需要很长的处理时间且化合物产率较低。当MMIPs作为固相萃取（SPE）吸附剂用于样品分离时，将MMIPs引入溶解的样品中并使其均匀分散，它们会在分析物和MMIPs之间形成键合，然后使用外部磁场将其与样品基质分离。这种从样品基质中分离分析物的过程使得使用MMIPs的分离技术非常简单易行。本文综述了如何合成MMIPs、其合成过程中必须考虑的因素以及它们在从天然产物中分离活性化合物方面的应用。由于合成产率高，通过共沉淀制备的具有磁性核壳结构的MMIPs可能是进一步发展的良好选择。进一步优化影响磁性核壳颗粒尺寸和分布的因素，可以获得具有更高吸附容量和选择性的MMIPs的更高合成产率。因此，它们能够以高收率和纯度从天然植物中分离目标化合物。
