College of Pharmacy, Institute of Medicine, Xi'an Medical University, Xi'an, 710021, China.
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Material Science, Northwest University, Xi'an, 710121, China.
Mikrochim Acta. 2019 Sep 16;186(10):683. doi: 10.1007/s00604-019-3785-y.
A boronate-modified magnetic affinity sorbent was prepared by adopting hyperbranched polyethyleneimine as the scaffold to amplify initiator sites. 3-Acrylamidophenylboronic acid was employed as monomer to proceed in situ free-radical polymerization on magnetite (FeO) nanoparticles. Due to the improved density of boronic acid polymers, the adsorbent exhibited high adsorption capacity, typically (134 ± 8) μg mg for dopamine, (92 ± 7) μg mg for catechol, (363 ± 14) μg mg for ovalbumin and (464 ± 22) μg mg for horseradish peroxidase. These capacities are much higher than those of other adsorbents. The sorbent was applied to the enrichment of catecholamines from spiked human urine. Owing to the high adsorption capacity, only 1.0 mg of adsorbent was sufficient to eliminate the interferences and enrich the targets (dopamine, norepinephrine and epinephrine) within 5 min. They were quantified by HPLC. The recoveries from spiked samples range between 83.5% ~106%, with relative standard deviations of 3.2 ~ 9.4% (n = 5). The separation of glycoproteins from egg white was also accomplished prior to their analysis by PAGE. In the authors' perception, this approach is promising in that the density of functional groups on the adsorbent is strongly increased. Graphical abstract The preparation routine of boronate affinity magnetic adsorbent (FeO@HpAAPBA). The adsorbent is used for the magnetic solid phase extraction of cis-dol compounds from real sample.
一种硼酸盐修饰的磁性亲和吸附剂是通过采用超支化聚乙烯亚胺作为支架来放大引发剂位点来制备的。3-丙烯酰胺基苯硼酸用作单体，在磁铁矿（FeO）纳米粒子上进行原位自由基聚合。由于硼酸盐聚合物的密度增加，该吸附剂表现出高的吸附容量，通常对多巴胺的吸附容量为（134±8）μg mg，对儿茶酚的吸附容量为（92±7）μg mg，对卵清蛋白的吸附容量为（363±14）μg mg，对辣根过氧化物酶的吸附容量为（464±22）μg mg。这些容量远高于其他吸附剂。该吸附剂用于从加标人尿中富集儿茶酚胺。由于高的吸附容量，仅需 1.0 mg 的吸附剂即可在 5 min 内消除干扰并富集目标物（多巴胺、去甲肾上腺素和肾上腺素）。通过 HPLC 进行定量。从加标样品中的回收率在 83.5%106%之间，相对标准偏差为 3.2%9.4%（n=5）。在进行 PAGE 分析之前，还完成了卵清白蛋白中糖蛋白的分离。在作者看来，这种方法很有前景，因为吸附剂上的官能团密度大大增加。
