Fong C C, Shi Y F, Yu W K, Wei F, van de Merwe J P, Chan Alice K Y, Ye R, Au Doris W T, Wu Rudolf S S, Yang M S
State Key Laboratory in Marine Pollution, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong; Shenzhen Key Laboratory of Biochip Research, City University of Hong Kong, Shenzhen 518057, PR China.
Shenzhen Key Laboratory of Biochip Research, City University of Hong Kong, Shenzhen 518057, PR China; Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Mar Pollut Bull. 2014 Aug 30;85(2):471-8. doi: 10.1016/j.marpolbul.2014.04.024. Epub 2014 May 19.
A recent study demonstrated that 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) may have an adverse effect on the reproduction in marine medaka (Oryzias melastigma), but the molecular mechanisms remain largely unknown. In this study, we investigated the protein expression profiles of male and female gonads of O. melastigma exposed to dietary BDE-47 at two dosages (0.65 and 1.30 μg/g/day, respectively) for 21 days. Extracted proteins were labeled with iTRAQ and analyzed on a MALDI TOF/TOF analyzer, as results, 133 and 144 unique proteins were identified in testis and ovary, respective, and they exerted dose- and sex-dependent expression patterns. In testis, among the 42 differentially expressed proteins; down-regulation of histone variants and parvalbumins implicated BDE-47 may disrupt the spermatogenesis and induce sterility in fishes. In ovary, 38 proteins were differentially expressed; the elevation of vitellogenins and apolipoprotein A-I expression indicated BDE-47 acts as an estrogen-mimicking compound and led to reproductive impairment in O. melastigma.
最近的一项研究表明，2,2',4,4'-四溴二苯醚（BDE-47）可能对海洋青鳉（Oryzias melastigma）的繁殖产生不利影响，但其分子机制仍 largely unknown。在本研究中，我们调查了分别以两种剂量（分别为0.65和1.30μg/g/天）的膳食BDE-47处理21天的青鳉雄性和雌性性腺的蛋白质表达谱。提取的蛋白质用iTRAQ标记并在MALDI TOF/TOF分析仪上进行分析，结果分别在睾丸和卵巢中鉴定出133和144种独特蛋白质，它们呈现出剂量和性别依赖性表达模式。在睾丸中，在42种差异表达的蛋白质中，组蛋白变体和小清蛋白下调表明BDE-47可能破坏鱼类的精子发生并导致不育。在卵巢中，有38种蛋白质差异表达；卵黄蛋白原和载脂蛋白A-I表达升高表明BDE-47作为一种雌激素模拟化合物导致青鳉的生殖损伤。
