Han Jing, Li Pao, Cai Wensheng, Shao Xueguang
Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), State Key Laboratory of Medicinal Chemical Biology, and Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin, China.
J Sep Sci. 2014 Aug;37(16):2126-30. doi: 10.1002/jssc.201400403. Epub 2014 Jun 20.
Ginseng is a well-known traditional Chinese medicinal herb, and ginsenosides are its major active components. A method for the fast determination of ginsenosides in ginseng samples by high-performance liquid chromatography was developed and used for the quantitative analysis of four ginsenosides in three different ginseng samples. In this method, instead of time-consuming gradient elution, isocratic elution was used to speed up the analysis. Under strong isocratic elution, all the ginsenosides are eluted in 2.3 min. Although the measured signal is composed of overlapped peaks with the interferences and background, the signal of ginsenosides can be extracted by chemometric resolution. A non-negative immune algorithm was employed to obtain the chromatographic information of the target components from the data. Compared with conventional chemometric approaches, the method can perform the extraction for one-dimensional overlapping signals. The method was validated by the determination of four ginsenosides in three different ginseng samples. The recoveries of the spiked samples were in the range of 94.08-107.3%.
人参是一种著名的传统中草药,人参皂苷是其主要活性成分。开发了一种通过高效液相色谱快速测定人参样品中人参皂苷的方法,并用于三种不同人参样品中四种人参皂苷的定量分析。在该方法中,采用等度洗脱而非耗时的梯度洗脱来加快分析速度。在强等度洗脱条件下,所有的人参皂苷在2.3分钟内被洗脱出来。尽管测得的信号由重叠峰以及干扰和背景组成,但人参皂苷的信号可通过化学计量学分辨来提取。采用非负免疫算法从数据中获取目标成分的色谱信息。与传统化学计量学方法相比,该方法可对一维重叠信号进行提取。通过测定三种不同人参样品中的四种人参皂苷对该方法进行了验证。加标样品的回收率在94.08%至107.3%之间。