胸腺素β10促进肺癌细胞中VEGF-C的表达。
[Thymosin beta 10 prompted the VEGF-C expression in lung cancer cell].
作者信息
Li Zixuan, Qu Lianyue, Zhong Hongshan, Xu Ke, Qiu Xueshan
机构信息
Department of Pathology, the First Affiliated Hospital of China Medical University and College of Basic Medical Sciences, China Medical University, Shenyang 110001, China;Department of Radiology and Key Laboratory of Diagnostic Imaging and Interventional Radiology of Liaoning Province, the First Affiliated Hospital of China Medical University, Shenyang 110001, China.
Department of Pharmacy, the First Affiliated Hospital of China Medical University, Shenyang 110001, China.
出版信息
Zhongguo Fei Ai Za Zhi. 2014 May;17(5):378-83. doi: 10.3779/j.issn.1009-3419.2014.05.03.
BACKGROUND AND OBJECTIVE
Our previous study found that thymosin β10 overexpressed in lung cancer and positively correlated with differentiation, lymph node metastasis and stage of lung cancer. In this reasearch we aim to study the effects and mechanism of exogenous human recombinant Tβ10 on the expression of VEGF-C on non-small cell lung cancer.
METHODS
After SPC, A549 and LK2 cells were treated with 100 ng/mL recombinant human Tβ10, the mRNA level of VEGF-C were detected by RT-PCR. The mean while the protein expression of VEGF-C, P-AKT and AKT were determined by Western blot assay.
RESULTS
Exogenous recombinant human Tβ10 were significantly promote the expression levels of VEGF-C mRNA and protein while promoting the phosphorylation of AKT. Exogenous Tβ10 can promote the expression of VEGF-C mRNA and protein in lung cancer cell lines A549 and LK2 (P<0.05), and this effect can be inhibited by use AKT inhibitor LY294002 (P<0.05).
CONCLUSIONS
Tβ10 human recombinant proteins can promote the expression of VEGF-C by activating AKT phosphorylation in lung cancer cell lines.
背景与目的
我们之前的研究发现，胸腺素β10在肺癌中过表达，且与肺癌的分化、淋巴结转移及分期呈正相关。在本研究中，我们旨在探讨外源性人重组Tβ10对非小细胞肺癌中VEGF-C表达的影响及机制。
方法
用100 ng/mL重组人Tβ10处理SPC、A549和LK2细胞后，通过RT-PCR检测VEGF-C的mRNA水平。同时，用蛋白质印迹法测定VEGF-C、P-AKT和AKT的蛋白表达。
结果
外源性重组人Tβ10显著促进VEGF-C mRNA和蛋白的表达水平，同时促进AKT的磷酸化。外源性Tβ10可促进肺癌细胞系A549和LK2中VEGF-C mRNA和蛋白的表达（P<0.05），而使用AKT抑制剂LY294002可抑制这种作用（P<0.05）。
结论
人重组Tβ10蛋白可通过激活肺癌细胞系中的AKT磷酸化来促进VEGF-C的表达。
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