Li Min, Zhang Yuqing, Zhai Qihui, Feurino Louis W, Fisher William E, Chen Changyi, Yao Qizhi
Molecular Surgeon Research Center, Elkins Pancreas Center, Michael E. DeBakey Department of Surgery, Baylor College of Medicine, Houston, Texas 77030, USA.
Cancer Invest. 2009 Mar;27(3):251-6. doi: 10.1080/07357900802254016.
Thymosin beta-10 (T beta 10) has been shown to be associated with several cancers; however, its role in pancreatic cancer is not understood. The expression of T beta 10 was determined by immunohistochemistry and real-time polymerase chain reaction. The phosphorylation of JNK and the cytokine secretion was determined by using the Bio-Plex phosphoprotein and cytokines assays. Pancreatic cancer tissues and cells expressed higher amounts of T beta 10 than normal surrounding tissues and human pancreatic duct epithelial cells. Exogenous T beta 10 caused the phosphorylation of JNK and increased the secretion of cytokines interleukin (IL)-7 and IL-8 in BxPC-3 cells. T beta 10 might be a promising marker and a novel therapeutic target for pancreatic cancer.
胸腺素β-10(Tβ10)已被证明与多种癌症相关;然而,其在胰腺癌中的作用尚不清楚。通过免疫组织化学和实时聚合酶链反应测定Tβ10的表达。使用生物芯片磷酸化蛋白和细胞因子检测法测定JNK的磷酸化和细胞因子分泌。胰腺癌组织和细胞中Tβ10的表达量高于周围正常组织和人胰腺导管上皮细胞。外源性Tβ10导致BxPC-3细胞中JNK磷酸化,并增加细胞因子白细胞介素(IL)-7和IL-8的分泌。Tβ10可能是一种有前景的胰腺癌标志物和新型治疗靶点。