Zanini Daniela, Pelinson Luana Paula, Schmatz Roberta, Belmonte Pereira Luciane, Curry Martins Caroline, Baldissareli Jucimara, Pires Amaral Guilherme, Antunes Soares Félix Alexandre, Brenner Reetz Luiz Gustavo, Araújo Maria do Carmo, Chiesa Juarez, Morsch Vera Maria, Bitencourt Rosa Leal Daniela, Schetinger Maria Rosa Chitolina
Programa de Pós-Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil.
Programa de Pós-Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil.
Biomed Pharmacother. 2014 Jun;68(5):603-9. doi: 10.1016/j.biopha.2014.04.005. Epub 2014 Apr 28.
This study investigated the δ-aminolevulinate dehydratase (δ-ALA-D) activity in whole blood as well as the parameters of oxidative stress, such as reactive species (RS) levels in serum, thiobarbituric acid reactive substances (TBARS) levels, the superoxide dismutase (SOD) and catalase (CAT) activities, as well as total thiols (T-SH) and non-protein thiols (NPSH) levels in platelets. Moreover, the content of vitamin C and E in plasma and serum, respectively, in lung cancer patients was also investigated. We collected blood samples from patients (n=28) previously treated for lung cancer with chemotherapy. Patients were classified as stage IIIb and IV according to the Union for International Cancer Control (UICC). Results showed a decrease of 37% in δ-ALA-D activity in patients with lung cancer when compared to the control group. RS and TBARS levels were 8% and 99% higher in the patient group, respectively. The activity of SOD and CAT as well as the vitamin C content were 41%, 35% and 127% lower in patients when compared with controls, respectively. However, T-SH and vitamin E levels were 27% and 44% higher in lung cancer patients, respectively. Results show that the overproduction of reactive species in patients with lung cancer may be interfering with the activity of δ-ALA-D. Likewise, the decrease in the activity of this enzyme may be contributing for the oxidative stress.
本研究调查了全血中δ-氨基乙酰丙酸脱水酶（δ-ALA-D）的活性以及氧化应激参数，如血清中活性物质（RS）水平、硫代巴比妥酸反应性物质（TBARS）水平、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性，以及血小板中总硫醇（T-SH）和非蛋白硫醇（NPSH）水平。此外，还分别研究了肺癌患者血浆和血清中维生素C和E的含量。我们收集了先前接受化疗治疗的肺癌患者（n = 28）的血样。根据国际癌症控制联盟（UICC），患者被分类为IIIb期和IV期。结果显示，与对照组相比，肺癌患者的δ-ALA-D活性降低了37%。患者组的RS和TBARS水平分别高出8%和99%。与对照组相比，患者的SOD和CAT活性以及维生素C含量分别降低了41%、35%和127%。然而，肺癌患者的T-SH和维生素E水平分别高出27%和44%。结果表明，肺癌患者活性物质的过度产生可能会干扰δ-ALA-D的活性。同样，这种酶活性的降低可能导致氧化应激。
