Polachini Carla Roberta Nunes, Spanevello Roselia Maria, Zanini Daniela, Baldissarelli Jucimara, Pereira Luciane Belmonte, Schetinger Maria Rosa Chitolina, da Cruz Ivana Beatrice Mânica, Assmann Charles Elias, Bagatini Margarete Dulce, Morsch Vera Maria
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, Santa Maria, RS, 97105-900, Brazil.
Programa de Pós-Graduação em Bioquímica e Bioprospecção, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Campus Universitário, Capão do Leão, Pelotas, RS, 96010-900, Brazil.
Neurotox Res. 2016 Feb;29(2):230-42. doi: 10.1007/s12640-015-9584-2. Epub 2015 Dec 21.
Multiple sclerosis (MS) is an autoimmune neurological disorder of unknown etiology. Oxidative stress and alterations in vitamin D levels have been implicated in the pathophysiology of MS. The aim of this study was to investigate δ-aminolevulinate dehydratase (δ-ALA-D) activity as well as the levels of vitamin D, lipid peroxidation levels, carbonyl protein content, DNA damage, superoxide dismutase (SOD) and catalase (CAT) activities, and the vitamin C, vitamin E, and non-protein thiol (NPSH) content in samples from patients with the relapsing-remitting form of MS (RRMS). The study population consisted of 29 RRMS patients and 29 healthy subjects. Twelve milliliters of blood was obtained from each individual and used for biochemical determinations. The results showed that δ-ALA-D and CAT activities were significantly increased, while SOD activity was decreased in the whole blood of RRMS patients compared to the control group (P < 0.05). In addition, we observed a significant increase in lipid peroxidation, carbonyl protein levels in serum and damaged DNA in leucocytes in RRMS patients compared with the control group (P < 0.05). Nonetheless, the levels of vitamin C, vitamin E, NPSH, and vitamin D were significantly decreased in RRMS patients in relation to the healthy individuals (P < 0.05). In conclusion, our results suggested that the increase in δ-ALA-D activity may be related to the inflammatory and immune process in MS in an attempt to maintain the cellular metabolism and reduce oxidative stress. Moreover, the alterations in the oxidant/antioxidant balance and lower vitamin D levels may contribute to the pathophysiology of MS.
多发性硬化症（MS）是一种病因不明的自身免疫性神经疾病。氧化应激和维生素D水平的改变与MS的病理生理学有关。本研究的目的是调查复发缓解型多发性硬化症（RRMS）患者样本中的δ-氨基乙酰丙酸脱水酶（δ-ALA-D）活性以及维生素D水平、脂质过氧化水平、羰基蛋白含量、DNA损伤、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性，以及维生素C、维生素E和非蛋白巯基（NPSH）含量。研究人群包括29例RRMS患者和29名健康受试者。从每个个体采集12毫升血液并用于生化测定。结果显示，与对照组相比，RRMS患者全血中的δ-ALA-D和CAT活性显著增加，而SOD活性降低（P < 0.05）。此外，我们观察到与对照组相比，RRMS患者的脂质过氧化、血清羰基蛋白水平和白细胞中受损DNA显著增加（P < 0.05）。尽管如此，RRMS患者的维生素C、维生素E、NPSH和维生素D水平相对于健康个体显著降低（P < 0.05）。总之，我们的结果表明，δ-ALA-D活性的增加可能与MS中的炎症和免疫过程有关，试图维持细胞代谢并减少氧化应激。此外，氧化/抗氧化平衡的改变和较低的维生素D水平可能有助于MS的病理生理学。
