Programa de Pós-Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Departamento de Química, Universidade Federal de Santa Maria, Campus Universitário, Camobi, Santa Maria, RS, 97105-900, Brazil.
Mol Cell Biochem. 2013 Feb;374(1-2):137-48. doi: 10.1007/s11010-012-1513-6. Epub 2012 Nov 18.
We aimed to examine the nucleoside triphosphate diphosphohydrolases (NTPDase) in lymphocytes; adenosine deaminase (ADA) and butyrylcholinesterase (BChE) in serum; and acetylcholinesterase (AChE), superoxide dismutase (SOD), and catalase (CAT) activity in whole blood; since these enzymes are involved in inflammation responses as well as in oxidative stress conditions. We also checked the levels of total thiols (T-SH), non-protein thiols (NPSH), and thiobarbituric acid reactive substances (TBARS) in serum of patients with lung cancer. We collected blood samples from patients (n = 31) previously treated for lung cancer with chemotherapy. Patients were classified as stage IIIb and IV according to the Union for International Cancer Control (UICC). The results showed a significant increase in the hydrolysis of ATP, ADP, and adenosine in patients when compared with the control group. The activity of AChE, SOD, and CAT as well as the T-SH and NPSH levels were higher in patients group and TBARS levels were lower in patients compared with the control group. These findings demonstrated that the enzymes activity involved in the control of inflammatory and immune processes as well as the oxidative stress parameters are altered in patients with lung cancer.
我们旨在研究淋巴细胞中的核苷三磷酸二磷酸水解酶（NTPDase）；血清中的腺苷脱氨酶（ADA）和丁酰胆碱酯酶（BChE）；以及全血中的乙酰胆碱酯酶（AChE）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性；因为这些酶参与炎症反应以及氧化应激条件。我们还检查了肺癌患者血清中的总巯基（T-SH）、非蛋白巯基（NPSH）和硫代巴比妥酸反应物质（TBARS）水平。我们从先前接受过肺癌化疗的患者（n=31）中采集了血液样本。根据国际癌症控制联盟（UICC），患者被分为 IIIb 期和 IV 期。结果显示，与对照组相比，患者体内 ATP、ADP 和腺苷的水解显著增加。患者组的 AChE、SOD 和 CAT 活性以及 T-SH 和 NPSH 水平较高，而 TBARS 水平较低。这些发现表明，参与控制炎症和免疫过程以及氧化应激参数的酶活性在肺癌患者中发生了改变。
