超顺磁性氧化铁标记对多发性硬化症患者来源的人骨髓间充质干细胞主要功能特性的影响。
Effects of supermagnetic iron oxide labeling on the major functional properties of human mesenchymal stem cells from multiple sclerosis patients.
机构信息
Department of Neurology, Laboratory of Neuroimmunology and Agnes Ginges Center for Neurogenetics and Multiple Sclerosis Center, Hadassah-Hebrew University Medical Center, Jerusalem, Israel.
National Institute of Sciences, Bethesda, USA.
出版信息
Int J Stem Cells. 2010 May;3(2):144-53. doi: 10.15283/ijsc.2010.3.2.144.
BACKGROUND AND OBJECTIVES
In the last few years, treatment protocols using mesenchymal stem cells (MSC) in various experimental models and human diseases have been investigated. MSCs are on the focus of stem cell research, since they are considered as a type of adult stem cells with low toxicity and acceptable side effects profile and they can be administered autologously. In addition several studies have revealed significant immunomodulatory properties of MSCs and a potential for transdifferentiation, including neural differentiation, both in vivo and in vitro. Magnetic resonance imaging (MRI) is a non-invasive technique that can be used to track labeled cells and evaluate their migration ability in various clinical settings.
METHODS AND RESULTS
In this study we investigated whether such labeling of MSCs with the commercially used para-magnetic material, Feridex, has any negative effect on the above mentioned functional properties of MSCs. We labeled human mesenchymal stem cells (hMSC) with poly-L-lysine coated Feridex(®) and evaluated their cellular differentiation and immunomodulatory properties, in vitro. In comparison with unlabeled cells, labeled hMSC exhibited normal adipogenic and osteogenic differentiation, but decreased chondrogenic differentiation. Regarding neural differentiation, labeled and unlabeled cells were similar in their ability to express neural-like and glial like surface proteins. Finally, both labeled and unlabeled MSCs exhibited a dose-dependent, significant blocking effect on the proliferation of healthy donors lymphocytes following mitogen stimulation.
CONCLUSIONS
These findings indicate that labeling with Feridex does not affect the immunomodulatory, nor the neural transdifferentiation potential of MScs and therefore, Feridex may be used for the tracking of this type of stem cells in clinical applications, without compromising their major functional properties.
背景与目的
在过去的几年中，人们研究了使用间充质干细胞（MSC）在各种实验模型和人类疾病中的治疗方案。MSC 是干细胞研究的焦点，因为它们被认为是一种具有低毒性和可接受的副作用特征的成体干细胞，并且可以自体给药。此外，几项研究表明 MSC 具有显著的免疫调节特性和潜在的转分化能力，包括体内和体外的神经分化。磁共振成像（MRI）是一种非侵入性技术，可用于跟踪标记的细胞并评估其在各种临床环境中的迁移能力。
方法和结果
在这项研究中，我们研究了用商业上使用的顺磁性材料 Feridex 标记 MSC 是否会对 MSC 的上述功能特性产生任何负面影响。我们用聚-L-赖氨酸包被的 Feridex（®）标记人骨髓间充质干细胞（hMSC），并在体外评估其细胞分化和免疫调节特性。与未标记的细胞相比，标记的 hMSC 表现出正常的成脂和成骨分化，但软骨分化减少。关于神经分化，标记和未标记的细胞在表达神经样和神经胶质样表面蛋白的能力上相似。最后，标记和未标记的 MSC 均表现出对有丝分裂原刺激后健康供体淋巴细胞增殖的剂量依赖性显著阻断作用。
结论
这些发现表明，Feridex 标记不会影响 MSC 的免疫调节或神经转分化潜能，因此，Feridex 可用于临床应用中追踪这种类型的干细胞，而不会损害其主要功能特性。
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