Thapsukhon Boontharika, Daranarong Donraporn, Meepowpan Puttinan, Suree Nuttee, Molloy Robert, Inthanon Kewalin, Wongkham Weerah, Punyodom Winita
J Biomater Sci Polym Ed. 2014 Jul;25(10):1028-44. doi: 10.1080/09205063.2014.918457. Epub 2014 May 23.
In this study, for the first time, a biodegradable poly(L-lactide-co-ε-caprolactone), PLC 67:33 copolymer was developed for use as temporary scaffolds in reconstructive nerve surgery. The effect of the surface topology and pore architecture were studied on the biocompatibility for supporting the growth of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (hWJ-MSCs) and human neuroblastoma cells (hNBCs) as cell models. Porous PLC membranes were prepared by electrospinning and phase immersion precipitation with particulate leaching and nonporous PLC membranes were prepared by solvent casting. From the results, the porous PLC membranes can support hWJ-MSCs and hNBCs cells better than the nonporous PLC membrane, and the interconnected pore scaffold prepared by electrospinning exhibited a more significant supporting attachment of the cells than the open pore and nonporous membranes. We can consider that these electrospun PLC membranes with 3-D interconnecting fiber networks and a high porosity warrant a potential use as nerve guides in reconstructive nerve surgery.
在本研究中，首次开发了一种可生物降解的聚（L-丙交酯-共-ε-己内酯），即PLC 67:33共聚物，用于重建神经手术中的临时支架。以人脐带华通氏胶来源的间充质干细胞（hWJ-MSCs）和人神经母细胞瘤细胞（hNBCs）作为细胞模型，研究了表面拓扑结构和孔隙结构对生物相容性的影响，以支持其生长。通过静电纺丝和颗粒沥滤相沉浸沉淀法制备多孔PLC膜，通过溶剂浇铸法制备无孔PLC膜。结果表明，多孔PLC膜比无孔PLC膜能更好地支持hWJ-MSCs和hNBCs细胞生长，且通过静电纺丝制备的相互连通的孔隙支架比开孔和无孔膜表现出更显著的细胞支持附着作用。我们可以认为，这些具有三维互连纤维网络和高孔隙率的静电纺丝PLC膜有望在重建神经手术中用作神经导管。
