Department of Internal Medicine, Fongyuan Hospital, Taichung, Taiwan.
Department of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung, Taiwan.
J Microbiol Immunol Infect. 2015 Oct;48(5):559-64. doi: 10.1016/j.jmii.2014.04.004. Epub 2014 May 22.
The clinical impact of Chryseobacterium indologenes infection is increasing; nevertheless, most studies had been conducted in northern Taiwan, but rarely in central Taiwan.
Using 16S rRNA gene sequencing, 34 isolates of C. indologenes were identified at the Central Region Hospital Alliance between 2007 and 2011. Vitek 2 and matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) methods were compared for the feasibility to identify this bacterium. Drug susceptibility test, biofilm formation, and pulsed-field gel electrophoresis (PFGE) were also performed.
All isolates were collected from hospitalized patients with an average age of 70.8 ± 18.5 years. The most prevalent sample was urine (50.0%), followed by sputum (32.4%). The accuracy rate of species-level identification reached 94.1% using the Vitek 2 method and 85.3% using the MALDI-TOF MS method. All of the isolates were resistant to gentamicin, amikacin, ceftriaxone, chloramphenicol, colistin, and imipenem, but completely susceptible to minocycline. While analyzing biofilm-forming ability, 38.2% (13/34) of C. indologenes isolates displayed a positive phenotype using the Luria-Bertani (LB) medium. However, 80.0% (4/5) of invasive isolates were biofilm producers. Based on PFGE analysis, several clusters were found, and the possible intrahospital spread of this bacterium in this area could not be excluded.
Both Vitek 2 and MALDI-TOF MS methods showed good ability in the determination of C. indologenes. Among the examined drugs, minocycline was the most potent one. As many as 38.2% C. indologenes isolates showed biofilm-forming ability. PFGE analyses revealed the possible intrahospital transmission of this bacterium in central Taiwan.
食酸菌属（Chryseobacterium）感染的临床影响正在增加；然而，大多数研究都在台湾北部进行，而在台湾中部则很少。
在 2007 年至 2011 年间，中部地区医院联盟使用 16S rRNA 基因测序鉴定了 34 株食酸菌。比较了 Vitek 2 和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）方法对该细菌鉴定的可行性。还进行了药敏试验、生物膜形成和脉冲场凝胶电泳（PFGE）。
所有分离株均从住院患者中采集，平均年龄为 70.8±18.5 岁。最常见的样本是尿液（50.0%），其次是痰液（32.4%）。Vitek 2 法鉴定种水平的准确率为 94.1%，MALDI-TOF MS 法为 85.3%。所有分离株均对庆大霉素、阿米卡星、头孢曲松、氯霉素、黏菌素和亚胺培南耐药，但对米诺环素完全敏感。在分析生物膜形成能力时，34 株食酸菌分离株中有 38.2%（13/34）在 LB 培养基中表现出阳性表型。然而，80.0%（4/5）的侵袭性分离株为生物膜生产者。根据 PFGE 分析，发现了几个聚类，不能排除该地区这种细菌在医院内传播的可能性。
Vitek 2 和 MALDI-TOF MS 两种方法均显示出鉴定食酸菌的良好能力。在所检查的药物中，米诺环素的作用最强。多达 38.2%的食酸菌分离株具有形成生物膜的能力。PFGE 分析显示，该菌在台湾中部可能存在医院内传播。
