Thesleff I, Vainio S, Jalkanen M
Department of Pedodontics and Orthodontics, University of Helsinki, Finland.
Int J Dev Biol. 1989 Mar;33(1):91-7.
A chain of reciprocal interactions between the epithelial and mesenchymal tissues regulates both morphogenesis and cell differentiation in the developing tooth. The very early interactions lead to budding of the oral epithelium and to the characteristic condensation of the neural crest-derived mesenchymal cells around the epithelial bud. During the bell stage of morphogenesis, the mesenchymal cells which are in contact with the dental epithelium differentiate into odontoblasts. In this reveiw article we summarize the results of our descriptive and experimental studies, which indicate that differentiation of the dental mesenchymal cells into odontoblasts, as well the condensation of dental mesenchymal cells at the bud stage, are regulated by interactions between the cell surface and the extracellular matrix. Transfilter studies where the dental epithelium and mesenchyme were cultured on opposite sides of Nuclepore filters, led to the hypothesis that the differentiation of dental mesenchymal cells into odontoblasts is triggered by interactions between the cell surface and the epithelial basement membrane matrix. Immunohistochemical localization of various matrix molecules showed that the matrix glycoproteins fibronectin and tenascin are accumulated in the dental basement membrane at the time of odontoblast differentiation. Fibronectin and tenascin are known to interact with each other, with other matrix molecules as well as with the cell surface, and also to influence cell shape. We suggest that fibronectin and tenascin are involved in the cell-matrix interaction which leads to the polarization and differentiation of odontoblasts.(ABSTRACT TRUNCATED AT 250 WORDS)
上皮组织和间充质组织之间的一系列相互作用调节着发育中牙齿的形态发生和细胞分化。早期的相互作用导致口腔上皮出芽，并使神经嵴来源的间充质细胞围绕上皮芽发生特征性聚集。在形态发生的钟状期，与牙上皮接触的间充质细胞分化为成牙本质细胞。在这篇综述文章中，我们总结了描述性和实验性研究的结果，这些结果表明牙间充质细胞向成牙本质细胞的分化以及在芽期牙间充质细胞的聚集是由细胞表面与细胞外基质之间的相互作用所调节的。在核孔滤膜两侧分别培养牙上皮和间充质的透膜实验，得出了牙间充质细胞向成牙本质细胞的分化是由细胞表面与上皮基底膜基质之间的相互作用所触发的这一假说。各种基质分子的免疫组织化学定位显示，在成牙本质细胞分化时，基质糖蛋白纤连蛋白和腱生蛋白在牙基底膜中积累。已知纤连蛋白和腱生蛋白彼此之间、与其他基质分子以及与细胞表面相互作用，并且还会影响细胞形状。我们认为纤连蛋白和腱生蛋白参与了导致成牙本质细胞极化和分化的细胞-基质相互作用。（摘要截短于250词）
