Mioso Roberto, Marante Francisco Javier Toledo, Laguna Irma Herrera Bravo de
Biotechnology Center - CBIOTEC, Universidade Federal da Paraíba - UFPB, Campus I - Cidade Universitária, 58051-900 João Pessoa, Paraíba, Brazil.
Department of Chemistry, Universidad de Las Palmas de Gran Canaria - ULPGC, Campus Universitario de Tafira - Edificio de Ciencias Básicas, 35017 Gran Canaria, Spain.
Rev Iberoam Micol. 2015 Jul-Sep;32(3):147-52. doi: 10.1016/j.riam.2014.01.004. Epub 2014 May 21.
The filamentous fungus Penicillium roqueforti is a well-known multifunctional cell factory of high added-value biomolecules.
The objective of this work was to carry out a detailed analysis of the metabolites present in the culture broth of a new marine-derived Penicillium roqueforti strain isolated in the Canary Islands, Spain.
The fungal biomass production was carried out in liquid-state fermentation, and after 10-12 days of incubation at 22-25°C, the supernatant mycelia was separated by filtration, and the culture broth (12l) was stored in a refrigerator at 4°C for a subsequent liquid-liquid extraction with dichloromethane (3×), in accordance with the modified Kupchan method. The volatile and semi-volatile organic compounds were separated by chromatography and analyzed using GC-MS and NMR spectroscopy analyses.
Several volatile organic compounds involved in the fatty acid pathway were identified: a terpenoid, a cyclic dipeptide, phthalates, and an alkyl adipate. In addition, three categories of non-volatile compounds (alkanes, fatty acids and 1-alkanols) were identified by spectroscopy. The results show that the fermented broth of this fungal strain has no mycotoxins under the culture conditions applied.
It is hoped that this chemo-specific information will offer critical input for improving the biotechnological applications of this filamentous fungus.
丝状真菌罗克福特青霉是一种著名的高附加值生物分子多功能细胞工厂。
本研究旨在对从西班牙加那利群岛分离出的一株新的海洋来源罗克福特青霉菌株培养液中的代谢产物进行详细分析。
在液态发酵中进行真菌生物量生产，在22 - 25°C培养10 - 12天后，通过过滤分离上清液菌丝体，将培养液（12升）储存在4°C冰箱中，随后按照改良的库普昌方法用二氯甲烷进行液 - 液萃取（3次）。通过色谱法分离挥发性和半挥发性有机化合物，并使用气相色谱 - 质谱联用仪（GC - MS）和核磁共振光谱分析进行分析。
鉴定出了几种参与脂肪酸途径的挥发性有机化合物：一种萜类化合物、一种环二肽、邻苯二甲酸盐和一种己二酸烷基酯。此外，通过光谱鉴定出了三类非挥发性化合物（烷烃、脂肪酸和1 - 链烷醇）。结果表明，在所用培养条件下，该真菌菌株的发酵液没有霉菌毒素。
希望这些化学特异性信息将为改善这种丝状真菌的生物技术应用提供关键依据。
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