细胞外信号调节激酶 5（ERK5）在正常和退变人髓核组织和细胞中的差异表达。

Differential expression of extracellular-signal-regulated kinase 5 (ERK5) in normal and degenerated human nucleus pulposus tissues and cells.

机构信息

Guangzhou Institute of Traumatic Surgery, The Fourth Affiliated Hospital of Medical College, Jinan University, Guangzhou 510220, China.

出版信息

Biochem Biophys Res Commun. 2014 Jul 11;449(4):466-70. doi: 10.1016/j.bbrc.2014.05.042. Epub 2014 May 21.

DOI:10.1016/j.bbrc.2014.05.042

PMID:24857985

Abstract

Extracellular-signal-regulated kinase 5 (ERK5) is a member of the mitogen-activated protein kinase (MAPK) family and regulates a wide variety of cellular processes such as proliferation, differentiation, necrosis, apoptosis and degeneration. However, the expression of ERK5 and its role in degenerated human nucleus pulposus (NP) is hitherto unknown. In this study, we observed the differential expression of ERK5 in normal and degenerated human nucleus pulposus tissues by using immunohistochemical staining and Western blot. Treatment of NP cells with Pro-inflammatory cytokine, TNF-α decreased ERK5 gene expression as well as NP marker gene expression; including the type II collagen and aggrecan. Suppression of ERK5 gene expression in NP cells by ERK5 siRNA resulted in decreased gene expression of type II collagen and aggrecan. Furthermore, inhibition of ERK5 activation by BIX02188 (5μM) decreased the gene expression of type II collagen and aggrecan in NP cells. Our results document the expression of ERK5 in degenerated nucleus pulposus tissues, and suggest a potential involvement of ERK5 in human degenerated nucleus pulposus.

摘要

细胞外信号调节激酶 5（ERK5）是丝裂原活化蛋白激酶（MAPK）家族的成员，调节多种细胞过程，如增殖、分化、坏死、凋亡和退化。然而，ERK5 的表达及其在退变人髓核（NP）中的作用尚不清楚。在这项研究中，我们通过免疫组织化学染色和 Western blot 观察了 ERK5 在正常和退变人髓核组织中的差异表达。促炎细胞因子 TNF-α处理 NP 细胞后，ERK5 基因表达以及 NP 标志物基因表达，包括 II 型胶原和聚集蛋白聚糖，均降低。用 ERK5 siRNA 抑制 NP 细胞中的 ERK5 基因表达导致 II 型胶原和聚集蛋白聚糖的基因表达降低。此外，ERK5 激活的抑制剂 BIX02188（5μM）降低了 NP 细胞中 II 型胶原和聚集蛋白聚糖的基因表达。我们的结果记录了 ERK5 在退变髓核组织中的表达，并提示 ERK5 可能参与了人退变髓核。