Yu Xin-Yi, Du Bei-Bei, Gao Zhi-Hong, Zhang Shi-Jie, Tu Xu-Tong, Chen Xiao-Yun, Zhang Zhen, Qu Shen-Chun
College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.
Mol Biol Rep. 2014 Aug;41(8):5273-86. doi: 10.1007/s11033-014-3399-8. Epub 2014 May 24.
MicroRNAs (miRNAs) are small non-coding RNAs, which silence target mRNA via cleavage or translational inhibition to function in regulating gene expression. MiRNAs act as important regulators of plant development and stress response. For understanding the role of miRNAs responsive to apple ring rot stress, we identified disease-responsive miRNAs using high-throughput sequencing in Malus × domestica Borkh.. Four small RNA libraries were constructed from two control strains in M. domestica, crabapple (CKHu) and Fuji Naga-fu No. 6 (CKFu), and two disease stress strains, crabapple (DSHu) and Fuji Naga-fu No. 6 (DSFu). A total of 59 miRNA families were identified and five miRNAs might be responsive to apple ring rot infection and validated via qRT-PCR. Furthermore, we predicted 76 target genes which were regulated by conserved miRNAs potentially. Our study demonstrated that miRNAs was responsive to apple ring rot infection and may have important implications on apple disease resistance.
微小RNA（miRNA）是一类小的非编码RNA，其通过切割或翻译抑制使靶标mRNA沉默，从而在调节基因表达中发挥作用。miRNA作为植物发育和应激反应的重要调节因子。为了了解响应苹果轮纹病胁迫的miRNA的作用，我们利用高通量测序技术在苹果（Malus × domestica Borkh.）中鉴定了响应病害的miRNA。从苹果属的两个对照菌株海棠（CKHu）和富士长富6号（CKFu）以及两个病害胁迫菌株海棠（DSHu）和富士长富6号（DSFu）构建了四个小RNA文库。共鉴定出59个miRNA家族，其中5个miRNA可能响应苹果轮纹病感染，并通过qRT-PCR进行了验证。此外，我们预测了76个可能受保守miRNA调控的靶基因。我们的研究表明，miRNA对苹果轮纹病感染有响应，可能对苹果抗病性具有重要意义。
