Ratajczak L, Prus-Głowacki W, Ratajczak W, Lehmann T
Faculty of Biology, Adam Mickiewicz University, Poznań, Poland.
Acta Biochim Pol. 1989;36(3-4):285-93.
Monospecific antisera against three glutamate dehydrogenase (GDH) subunits of lupin root nodules were obtained. The use of sensitive mixed rocket immunoelectrophoresis enabled detection of seven GDH forms at the early stage of nodule development, thus providing evidence for the earlier hypothesized (L. Ratajczak et al., 1986, Physiol. Plant., 67, 685-689) random association of subunits 2g and 2a to form the remaining five GDH forms. All seven forms were localized in mitochondria. Immunological similarity was found between form 1 and plastid GDH.
获得了针对羽扇豆根瘤中三种谷氨酸脱氢酶（GDH）亚基的单特异性抗血清。使用灵敏的混合火箭免疫电泳能够在根瘤发育早期检测到七种GDH形式，从而为早期假设（L. Ratajczak等人，1986年，《植物生理学》，67卷，685 - 689页）的亚基2g和2a随机结合形成其余五种GDH形式提供了证据。所有七种形式都定位于线粒体中。在形式1和质体GDH之间发现了免疫相似性。
