Strózycki P, Legocki A B
Department of Biochemistry, University of Agriculture, Poznań, Poland.
Acta Biochim Pol. 1988;35(1):39-50.
A group of root nodule-specific plant proteins (nodulins) has been isolated from yellow lupin (Lupinus luteus) by immunoaffinity chromatography. The cytoplasmic nodule protein extract was initially enriched in nodulins on a column with immobilized IgG fraction. It was then purified by chromatography on Sepharose 4B - bound IgG against uninfected root proteins and finally on Sepharose 4B - bound IgG against Rhizobium lupini proteins. Rocket immunoelectrophoresis showed that the nodulin preparation did not react with antibodies against root or bacterial proteins. SDS gel electrophoresis of lupin nodulins revealed at least 23 polypeptides ranging in Mr, from 7,000 to 70,000, probably representing protein subunits.
通过免疫亲和色谱法从黄羽扇豆（Lupinus luteus）中分离出了一组根瘤特异性植物蛋白（根瘤蛋白）。细胞质根瘤蛋白提取物最初在固定化IgG组分的柱上进行根瘤蛋白富集。然后通过在与未感染根蛋白结合的琼脂糖4B-IgG上进行色谱分离，最后在与羽扇豆根瘤菌蛋白结合的琼脂糖4B-IgG上进行色谱分离来进行纯化。火箭免疫电泳表明，根瘤蛋白制剂与抗根或细菌蛋白的抗体不发生反应。羽扇豆根瘤蛋白的SDS凝胶电泳显示至少有23种多肽，其分子量在7000至70000之间，可能代表蛋白质亚基。
