Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Gastroenterol Res Pract. 2014;2014:964071. doi: 10.1155/2014/964071. Epub 2014 Apr 10.
Objective. The aims of this study were to investigate the effects of synchronized dual pulse gastric electrical stimulation (SGES) on gastric motility in different periods for diabetic rats and try to explore the possible mechanisms of the effects. Methods. Forty-six rats were used in the study. Gastric slow waves were recorded at baseline, 7-14-day diabetes and 56-63-day diabetes before and after stimulation and the age-matched control groups. SGES-60 mins and SGES-7 days (60 mins/day) were performed to test the effects on gastric motility and to evaluate glial marker S100B expression in stomach. Results. (1) Gastric emptying was accelerated in 7-14-day diabetes and delayed in 56-63-day diabetes. (2) The S100B expression in 56-63-day diabetes decreased and the ultrastructure changed. (3) The age-associated loss of EGC was observed in 56-63-day control group. (4) SGES was able to not only accelerate gastric emptying but also normalize gastric slow waves. (5) The S100B expression increased after SGES and the ultrastructure of EGC was partially restored. The effect of SGES-7 days was superior to SGES-60 mins. Conclusions. Delayed gastric emptying due to the growth of age may be related to the EGC inactivation. The effects of the SGES on gastric motility may be associated with EGC activation.
目的。本研究旨在探讨同步双脉冲胃电刺激（SGES）对糖尿病大鼠不同时期胃动力的影响，并试图探讨其可能的作用机制。
方法。本研究共纳入 46 只大鼠。在刺激前和刺激后，分别记录基础状态、7-14 天糖尿病和 56-63 天糖尿病大鼠以及年龄匹配的对照组大鼠的胃慢波。进行 SGES-60 分钟和 SGES-7 天（每天 60 分钟）以测试对胃动力的影响，并评估胃内神经胶质细胞标志物 S100B 的表达。
结果。（1）7-14 天糖尿病大鼠胃排空加速，56-63 天糖尿病大鼠胃排空延迟。（2）56-63 天糖尿病大鼠 S100B 表达降低，超微结构改变。（3）56-63 天对照组大鼠观察到与年龄相关的 EGC 丢失。（4）SGES 不仅能加速胃排空，还能使胃慢波正常化。（5）SGES 后 S100B 表达增加，EGC 的超微结构部分恢复。SGES-7 天的效果优于 SGES-60 分钟。
结论。由于年龄增长导致的胃排空延迟可能与 EGC 失活有关。SGES 对胃动力的影响可能与 EGC 激活有关。
