来自巴西里约热内卢的一只自然感染犬体内巴西利什曼原虫（维安尼亚利什曼原虫）和恰加斯利什曼原虫（利什曼原虫）的混合感染

Mixed infection with Leishmania (Viannia) braziliensis and Leishmania (Leishmania) chagasi in a naturally infected dog from Rio de Janeiro, Brazil.

作者信息

Madeira M F, Schubach A, Schubach T M P, Pacheco R S, Oliveira F S, Pereira S A, Figueiredo F B, Baptista C, Marzochi M C A

机构信息

Serviço de Parasitologia, Instituto de Pesquisa Clínica Evandro Chagas--FIOCRUZ, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brazil.

出版信息

Trans R Soc Trop Med Hyg. 2006 May;100(5):442-5. doi: 10.1016/j.trstmh.2005.07.011. Epub 2005 Oct 27.

DOI:10.1016/j.trstmh.2005.07.011

PMID:16257024

Abstract

We report here the first case of co-infection with Leishmania (Viannia) braziliensis and Leishmania (Leishmania) chagasi in a naturally infected dog from Rio de Janeiro, Brazil. Isoenzyme characterisation identified the parasites isolated in culture from the cutaneous lesion as L. (V.) braziliensis and the isolates from blood and lymph node as L. (L.) chagasi. PCR analysis using specific primers followed by molecular hybridisation for direct Leishmania species identification in tissue fragments confirmed the presence of L. (V.) braziliensis DNA in the cutaneous lesion and of L. (L.) chagasi DNA in spleen and popliteal lymph node fragments. This report emphasises the importance of identification of Leishmania species infecting seropositive dogs in endemic areas, and the consequent re-assessment of control and epidemiological surveillance measures for the control of leishmaniasis, as is the case in Brazil.

摘要

我们在此报告巴西里约热内卢一只自然感染犬同时感染巴西利什曼原虫（维安尼亚利什曼原虫）和恰加斯利什曼原虫（利什曼原虫）的首例病例。同工酶特征鉴定表明，从皮肤病变培养物中分离出的寄生虫为巴西利什曼原虫（维安尼亚利什曼原虫），而从血液和淋巴结中分离出的为恰加斯利什曼原虫（利什曼原虫）。使用特异性引物进行PCR分析，随后进行分子杂交以直接鉴定组织片段中的利什曼原虫种类，结果证实皮肤病变中存在巴西利什曼原虫（维安尼亚利什曼原虫）DNA，脾脏和腘淋巴结片段中存在恰加斯利什曼原虫（利什曼原虫）DNA。本报告强调了在流行地区鉴定感染血清阳性犬的利什曼原虫种类的重要性，以及因此对利什曼病控制和流行病学监测措施进行重新评估的必要性，巴西的情况就是如此。