Tan YongAn, Xiao LiuBin, Sun Yang, Zhao Jing, Bai LiXin
Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Pestic Biochem Physiol. 2014 May;111:43-50. doi: 10.1016/j.pestbp.2014.04.001. Epub 2014 Apr 18.
Hexaflumuron is a type of benzoylphenylurea insecticide which is highly toxic for many insects. Sublethal doses of hexaflumuron have been shown to significantly affect insect growth and development. However, the action mechanism of hexaflumuron is not well understood. In the present study, first instar Apolygus lucorum nymphs were exposed to sublethal doses of hexaflumuron based on the estimated 120h acute LC50 valve of 20.53mg/ml. We found that exposure to sublethal hexaflumuron doses resulted in a significant increase in development time and reduced the weights of fifth instar A. lucorum nymphs. We also measured trehalose, which is a primary blood sugar in insects, and the enzyme trehalase that is involved in energy metabolism. Trehalose content in first instar nymphs significantly increased following hexaflumuron treatment while the glucose content, soluble trehalase activity and expression levels of ALTre-1 mRNA decreased significantly. However, no significant changes in membrane-bound trehalase activity and ALTre-2 mRNA expression were observed. In addition, these decreases or increases could be correlated to increases in treatment time or concentration of hexaflumuron, respectively. The present findings indicated that sublethal doses of hexaflumuron could interfere the normal carbohydrate metabolism by depressing the expression of ALTre-1 in A. lucorum, which provide valuable information on the physiology and molecular mechanisms for the toxicity of hexaflumuron.
氟铃脲是一种对许多昆虫具有高毒性的苯甲酰基苯基脲类杀虫剂。已表明亚致死剂量的氟铃脲会显著影响昆虫的生长和发育。然而，氟铃脲的作用机制尚不清楚。在本研究中，根据估计的120小时急性LC50值20.53mg/ml，将一龄绿盲蝽若虫暴露于亚致死剂量的氟铃脲中。我们发现，暴露于亚致死剂量的氟铃脲会导致发育时间显著延长，并降低五龄绿盲蝽若虫的体重。我们还测量了昆虫的主要血糖海藻糖以及参与能量代谢的海藻糖酶。氟铃脲处理后，一龄若虫中的海藻糖含量显著增加，而葡萄糖含量、可溶性海藻糖酶活性和ALTre-1 mRNA的表达水平显著降低。然而，未观察到膜结合海藻糖酶活性和ALTre-2 mRNA表达的显著变化。此外，这些降低或增加可能分别与氟铃脲处理时间或浓度的增加相关。本研究结果表明，亚致死剂量的氟铃脲可通过抑制绿盲蝽中ALTre-1的表达来干扰正常的碳水化合物代谢，这为氟铃脲毒性的生理和分子机制提供了有价值的信息。
