Huynh Frank K, Green Michelle F, Koves Timothy R, Hirschey Matthew D
Duke Molecular Physiology Institute, Duke University Medical Center, Durham, North Carolina, USA.
Duke Molecular Physiology Institute, Duke University Medical Center, Durham, North Carolina, USA; Sarah W. Stedman Nutrition and Metabolism Center, Duke University Medical Center, Durham, North Carolina, USA; Department of Medicine, Duke University Medical Center, Durham, North Carolina, USA.
Methods Enzymol. 2014;542:391-405. doi: 10.1016/B978-0-12-416618-9.00020-0.
While much oncological research has focused on metabolic shifts in glucose and amino acid oxidation, recent evidence suggests that fatty acid oxidation (FAO) may also play an important role in the metabolic reprogramming of cancer cells. Here, we present a simple method for measuring FAO rates using radiolabeled palmitate, common laboratory reagents, and standard supplies. This protocol is broadly applicable for measuring FAO rates in cultured cancer cells as well as in both malignant and nontransformed animal tissues.
虽然许多肿瘤学研究都集中在葡萄糖和氨基酸氧化的代谢变化上，但最近的证据表明，脂肪酸氧化（FAO）在癌细胞的代谢重编程中也可能发挥重要作用。在此，我们介绍一种使用放射性标记的棕榈酸、常见实验室试剂和标准耗材测量FAO速率的简单方法。该方案广泛适用于测量培养的癌细胞以及恶性和未转化动物组织中的FAO速率。
