Hibberson C E, Vogelnest L J
University Veterinary Teaching Hospital Camden, University of Sydney, 410 Werombi Rd, Camden, New South Wales, 2570, Australia.
Aust Vet J. 2014 Jun;92(6):219-24. doi: 10.1111/avj.12185.
To evaluate contamination of unopened and opened stored sources of commercial dry dog food by viable storage mites.
Prospective laboratory and field study.
Samples were collected from nine brands of previously unopened bags (new bags) of dry food and 20 field sources of stored dry food in homes in Sydney and Canberra, Australia. All samples were initially examined for the presence of mites using a stereo-binocular microscope and then placed in separate filter-paper-sealed containers. Field samples were incubated at an average temperature of 29°C and 78% relative humidity (RH) for 5 weeks and then at average 26°C/83% RH for 8 weeks. Paired new-bag samples were stored under room conditions (average 23°C/47% RH) and controlled incubator conditions (average 26°C/80% RH) for 6 weeks. All samples were thoroughly examined for mites, mite eggs and visible mould once weekly using a stereo-binocular microscope.
Storage mites were not visualised in any of the field samples or in new-bag samples stored at room temperature. Storage mites, identified as Tyrophagus putrescentiae, were visualised in increasing numbers in seven of nine new-bag samples after incubation, with first mites and then eggs evident after 3 weeks of incubation.
We confirmed the presence of viable storage mites in a range of previously unopened commercial dry dog foods in Australia and confirmed the possibility of heavy storage mite contamination for dry food stored under conditions of moderate temperature and high humidity. These findings have relevance to storage mite and/or dust mite sensitivity in canine atopic dermatitis.
评估商业干狗粮未开封和已开封储存源被活的储存螨污染的情况。
前瞻性实验室和现场研究。
从澳大利亚悉尼和堪培拉家中的9个品牌先前未开封的干狗粮袋（新袋）以及20个储存干狗粮的现场来源采集样本。所有样本最初使用体视显微镜检查是否存在螨虫，然后放入单独的用滤纸密封的容器中。现场样本在平均温度29°C和相对湿度78%（RH）下孵育5周，然后在平均26°C/83%RH下孵育8周。成对的新袋样本在室温条件（平均23°C/47%RH）和可控培养箱条件（平均26°C/80%RH）下储存6周。每周使用体视显微镜对所有样本进行一次螨虫、螨卵和可见霉菌的彻底检查。
在任何现场样本或室温下储存的新袋样本中均未发现储存螨。在孵育后的9个新袋样本中的7个样本中，被鉴定为腐食酪螨的储存螨数量不断增加，孵育3周后首先出现螨虫，然后出现螨卵。
我们证实了在澳大利亚一系列先前未开封的商业干狗粮中存在活的储存螨，并证实了在中等温度和高湿度条件下储存的干狗粮有被大量储存螨污染的可能性。这些发现与犬特应性皮炎中对储存螨和/或尘螨的敏感性有关。
