Gill Christina, McEwan Neil, McGarry John, Nuttall Tim
The University of Liverpool School of Veterinary Science, Leahurst Campus, Neston, UK.
Vet Dermatol. 2011 Apr;22(2):162-72. doi: 10.1111/j.1365-3164.2010.00931.x. Epub 2010 Nov 25.
Dry pet food is a potential source of exposure to house dust and storage mite allergens in canine atopic dermatitis. This study evaluated contamination of house dust and dry dog food stored in paper bags, sealable plastic bags and sealable plastic boxes in 10 households for 90 days using Acarex(®) tests for guanine, a Der p 1 ELISA and mite flotation. Acarex(®) tests were negative in all the food samples but positive in all the house dust samples. The Der p 1 levels and mite numbers significantly increased in food from paper bags (P = 0.0073 and P = 0.02, respectively), but not plastic bags or boxes. Mite numbers and Der p 1 levels were 10-1000 times higher in house dust than the corresponding food samples (P < 0.0001). There were significant correlations between Der p 1 in house dust and food from the paper (P < 0.0001) and plastic bags (P = 0.003), and mite numbers in house dust and food from the paper bags (P = 0.0007). Bedding and carpets were significantly associated with Der p 1 levels in house dust (P = 0.015 and P = 0.01, respectively), and food from the paper (both P = 0.02) and plastic bags (P = 0.03 and P = 0.04, respectively). Mites were identified in six of 10 paper bag, three of 10 plastic bag, one of 10 plastic box and nine of 10 house dust samples. These comprised Dermatophagoides (54%), Tyrophagus (10%; all from food) and unidentified mites (36%). Storage of food in sealable plastic boxes largely prevented contamination for 3 months. Exposure to mites and mite proteins in all the stored food, however, appeared to be trivial compared with house dust.
干宠物食品是犬特应性皮炎中屋尘和储存螨过敏原的一个潜在暴露源。本研究使用Acarex（®）鸟嘌呤检测、Der p 1酶联免疫吸附测定（ELISA）和螨浮选法，对10户家庭中用纸质袋、可密封塑料袋和可密封塑料盒储存90天的屋尘和干狗粮的污染情况进行了评估。所有食品样本的Acarex（®）检测均为阴性，但所有屋尘样本均为阳性。纸质袋中的食品中Der p 1水平和螨数量显著增加（分别为P = 0.0073和P = 0.02），但塑料袋或塑料盒中的食品则未增加。屋尘中的螨数量和Der p 1水平比相应的食品样本高10至1000倍（P < 0.0001）。屋尘中的Der p 1与纸质袋（P < 0.0001）和塑料袋（P = 0.003）中的食品之间，以及屋尘中的螨数量与纸质袋中的食品之间（P = 0.0007）存在显著相关性。床上用品和地毯与屋尘中的Der p 1水平显著相关（分别为P = （此处原文可能有误，推测为0.015）和P = 0.01），以及与纸质袋（两者均为P = 0.02）和塑料袋中的食品相关（分别为P = 0.03和P = 0.04）。在10个纸质袋样本中有6个、10个塑料袋样本中有3个、10个塑料盒样本中有1个以及10个屋尘样本中有9个检测到螨。这些螨包括嗜皮螨属（54%）、食酪螨属（10%；均来自食品）和未鉴定的螨（36%）。将食品储存在可密封塑料盒中在很大程度上可防止3个月的污染。然而，与屋尘相比，所有储存食品中螨和螨蛋白的暴露似乎微不足道。
