来自经巴氏白蛉传播的两种利什曼原虫——图兰利什曼原虫和硕大利什曼原虫的脂磷壁酸的结构比较
Structural comparison of lipophosphoglycan from Leishmania turanica and L. major, two species transmitted by Phlebotomus papatasi.
作者信息
Volf Petr, Nogueira Paula M, Myskova Jitka, Turco Salvatore J, Soares Rodrigo P
机构信息
Department of Parasitology, Faculty of Science, Charles University, Prague 2 128 44, Czech Republic.
Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, 30190-002 Belo Horizonte, MG, Brazil.
出版信息
Parasitol Int. 2014 Oct;63(5):683-6. doi: 10.1016/j.parint.2014.05.004. Epub 2014 May 23.
The lipophosphoglycan (LPG) of Leishmania major has a major role in the attachment to Phlebotomus papatasi midgut. Here, we investigated the comparative structural features of LPG of L. turanica, another species transmitted by P. papatasi. The mAb WIC 79.3, specific for terminal Gal(β1,3) side-chains, strongly reacted with L. turanica LPG. In contrast, L. turanica LPG was not recognized by arabinose-specific mAb 3F12. In conclusion, LPGs from L. major and L. turanica are similar, with the latter being less arabinosylated than L. major's. The high galactose content in L. turanica LPG is consistent with its predicted recognition by P. papatasi lectin PpGalec.
硕大利什曼原虫的脂磷壁酸聚糖（LPG）在附着于巴氏白蛉中肠过程中起主要作用。在此，我们研究了由巴氏白蛉传播的另一个物种——图兰利什曼原虫LPG的比较结构特征。对末端Gal(β1,3)侧链具有特异性的单克隆抗体WIC 79.3与图兰利什曼原虫LPG强烈反应。相比之下，阿拉伯糖特异性单克隆抗体3F12不能识别图兰利什曼原虫LPG。总之，硕大利什曼原虫和图兰利什曼原虫的LPG相似，后者的阿拉伯糖基化程度低于硕大利什曼原虫。图兰利什曼原虫LPG中高含量的半乳糖与其被巴氏白蛉凝集素PpGalec识别的预测结果一致。
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