Rani Pittu Sandhya, Doddam Sankara Narayana, Agrawal Sashank, Hasnain Seyed E, Sechi Leonardo A, Kumar Ashutosh, Ahmed Niyaz
Pathogen Biology Laboratory, Department of Biotechnology and Bioinformatics, University of Hyderabad, Hyderabad, India.
Kusuma School of Biological Sciences, Indian Institute of Technology, New Delhi, India.
Int J Med Microbiol. 2014 Jul;304(5-6):620-5. doi: 10.1016/j.ijmm.2014.04.010. Epub 2014 May 5.
Mycobacterium avium ssp. paratuberculosis (MAP) is an obligate intracellular pathogen. It causes chronic intestinal inflammation in ruminants known as Johne's disease and is associated with human Crohn's disease. Furthermore, association of MAP with other autoimmune diseases, such as type-1 diabetes, has been established in patients from Sardinia (Italy) which is a MAP endemic and genetically isolated region. Due to largest livestock population and consequently high MAP prevalence amidst a very high diabetes incidence in India, we sought to test this association on a limited number of patient samples from Hyderabad. Our results of ELISA with MAP lysate and MAP-specific protein MAP3738c as well as PCR/real-time PCR of MAP-specific sequences IS900 and/or f57 indicated that, in contrast to Sardinian diabetic patients, MAP infection in blood is not discerned in diabetic patients in Hyderabad. The association of a mycobacterial trigger with diabetes therefore could well be a population-specific phenomenon, highly dependent on genetic repertoire and the environment of susceptible populations. However, a larger study is needed in order to confirm this.
副结核分枝杆菌鸟亚种（MAP）是一种专性细胞内病原体。它会在反刍动物中引发称为副结核病的慢性肠道炎症，并且与人类克罗恩病有关。此外，在意大利撒丁岛（一个MAP地方病流行且基因隔离的地区）的患者中，已证实MAP与其他自身免疫性疾病（如1型糖尿病）有关联。由于印度拥有庞大的牲畜种群，因此MAP流行率很高，同时糖尿病发病率也非常高，我们试图在海得拉巴的有限数量患者样本上检验这种关联。我们使用MAP裂解物和MAP特异性蛋白MAP3738c进行ELISA检测的结果，以及对MAP特异性序列IS900和/或f57进行PCR/实时PCR检测的结果表明，与撒丁岛糖尿病患者不同，在海得拉巴的糖尿病患者血液中未检测到MAP感染。因此，分枝杆菌引发因素与糖尿病之间的关联很可能是一种特定人群现象，高度依赖于易感人群的基因库和环境。然而，需要开展更大规模的研究来证实这一点。
