Park Jonghee, Byun Aram, Kim Do-Hoon, Shin Song Seok, Kim Jong-Ho, Kim Jin Woong
Dept. of Bionano Technology, Hanyang University, Ansan 426-791, Republic of Korea.
Amore-Pacific Co. R&D Center, Yongin 446-729, Republic of Korea.
J Colloid Interface Sci. 2014 Jul 15;426:162-9. doi: 10.1016/j.jcis.2014.03.058. Epub 2014 Apr 13.
This study introduces a flexible and straightforward method for generating monodisperse complex hydrogel microparticles. For this, a water-in-oil emulsion was generated in a microcapillary device and then the emulsion drops were photo-polymerized to transfer them to hydrogel particles. The hydrogel microparticles were made of poly (2-methacryloyloxyethyl phosphorylcholine) that has an enhanced biocompatibility due to the phosphatidyl choline moiety in the backbone. The average mesh size of the hydrogel network, which is 50Å, was estimated on the basis of the Peppas-Merrill equation. This mesh size was experimentally confirmed again by NMR cryoporometry analysis and permeation test for dextran probes. Furthermore, to diversify the applicability of microfluidic technology, an oil-in-water-in-oil double emulsion was also fabricated by using the co-axial jetting of three combined flows in the micro-channel. Then the aqueous shell was polymerized to give rise to hollow-structured hydrogel microparticles. Finally, we have shown that the microfluidic approach is useful for fabrication of complex hydrogel microparticles that have potential uses in drug immobilization and delivery.
本研究介绍了一种灵活且简便的方法来生成单分散复合水凝胶微粒。为此，在微毛细管装置中生成油包水乳液，然后将乳液滴进行光聚合，使其转化为水凝胶颗粒。水凝胶微粒由聚（2-甲基丙烯酰氧基乙基磷酰胆碱）制成，由于主链中的磷脂酰胆碱部分，其具有增强的生物相容性。基于Peppas-Merrill方程估算了水凝胶网络的平均网孔尺寸为50Å。通过核磁共振低温孔隙率分析和葡聚糖探针渗透试验再次对该网孔尺寸进行了实验验证。此外，为了使微流控技术的适用性多样化，还通过在微通道中对三种组合流进行同轴喷射制备了水包油包水双重乳液。然后使水相壳层聚合，得到中空结构的水凝胶微粒。最后，我们证明了微流控方法对于制备在药物固定和递送方面具有潜在用途的复合水凝胶微粒是有用的。
